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  Increased efficiency of charge-mediated fusion in polymer/lipid hybrid membranes

Marušič, N., Otrin, L., Rauchhaus, J., Zhao, Z., Kyrilis, F. L., Hamdi, F., et al. (2022). Increased efficiency of charge-mediated fusion in polymer/lipid hybrid membranes. Proceedings of the National Academy of Sciences of the United States of America, 119(20): e2122468119. doi:10.1073/pnas.2122468119.

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 Creators:
Marušič, Nika, Author
Otrin, Lado, Author
Rauchhaus, Jonas, Author
Zhao, Ziliang1, Author              
Kyrilis, Fotis L., Author
Hamdi, Farzad, Author
Kastritis, Panagiotis L., Author
Dimova, Rumiana1, Author              
Ivanov, Ivan, Author
Sundmacher, Kai, Author
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              

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Free keywords: polymer; phospholipid; fusion; membrane mixing; ATP
 Abstract: Due to their augmented properties, biomimetic polymer/lipid hybrid compartments are a promising substitute for natural liposomes in multiple applications, but the protein-free fusion of those semisynthetic membranes is unexplored to date. Here, we study the charge-mediated fusion of hybrid vesicles composed of poly(dimethylsiloxane)-graft-poly(ethylene oxide) and different lipids and analyze the process by size distribution and the mixing of membrane species at μm and nano scales. Remarkably, the membrane mixing of oppositely charged hybrids surpasses by far the degree in liposomes, which we correlate with properties like membrane disorder, rigidity, and ability of amphiphiles for flip-flop. Furthermore, we employ the integration of two respiratory proteins as a functional content mixing assay for different membrane compositions. This reveals that fusion is also attainable with neutral and cationic hybrids and that the charge is not the sole determinant of the final adenosine triphosphate synthesis rate, substantiating the importance of reconstitution environment. Finally, we employ this fusion strategy for the delivery of membrane proteins to giant unilamellar vesicles as a way to automate the assembly of synthetic cells.

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Language(s): eng - English
 Dates: 2022-05-122022
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1073/pnas.2122468119
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 119 (20) Sequence Number: e2122468119 Start / End Page: - Identifier: ISSN: 0027-8424