English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  The Sporisorium reilianum Effector Vag2 Promotes Head Smut Disease via Suppression of Plant Defense Responses

Zhao, Y., Agrawal, N., Ghareeb, H., Habib, M. T., Dickmeis, S., Schwachtje, J., et al. (2022). The Sporisorium reilianum Effector Vag2 Promotes Head Smut Disease via Suppression of Plant Defense Responses. Journal of Fungi, 8(5): 498. doi:10.3390/jof8050498.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
Link (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Zhao, Yulei1, Author
Agrawal, Nisha1, Author
Ghareeb, Hassan1, Author
Habib, Mohammad Tanbir1, Author
Dickmeis, Sascha1, Author
Schwachtje, J.2, Author              
Iven, Tim E.1, Author
Kopka, J.2, Author              
Feussner, Ivo1, Author
Schirawski, Jan1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              

Content

show
hide
Free keywords: -
 Abstract: Genome comparison between the maize pathogens Ustilago maydis and Sporisorium reilianum revealed a large diversity region (19-1) containing nearly 30 effector gene candidates, whose deletion severely hampers virulence of both fungi. Dissection of the S. reilianum gene cluster resulted in the identification of one major contributor to virulence, virulence-associated gene 2 (vag2; sr10050). Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) experiments revealed high expression of vag2 during biotrophic growth of S. reilianum. Using the yeast secretion trap assay, we confirmed the existence of a functional signal peptide allowing protein secretion via the conventional secretory pathway. We identified the cytoplasmic maize chorismate mutase ZmCM2 by yeast two-hybrid screening as a possible interaction partner of Vag2. Interaction of the two proteins in planta was confirmed by bimolecular fluorescence complementation. qRT-PCR experiments revealed vag2-dependent downregulation of salicylic acid (SA)-induced genes, which correlated with higher SA levels in plant tissues colonized by Delta;vag2 deletion strains relative to S. reilianum wildtype strains. Metabolite analysis suggested rewiring of pathogen-induced SA biosynthesis by preferential conversion of the SA precursor chorismate into the aromatic amino acid precursor prephenate by ZmCM2 in the presence of Vag2. Possibly, the binding of Vag2 to ZmCM2 inhibits the back reaction of the ZmCM2-catalyzed interconversion of chorismate and prephenate, thus contributing to fungal virulence by lowering the plant SA-induced defenses.

Details

show
hide
Language(s):
 Dates: 2022-05-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.3390/jof8050498
BibTex Citekey: jof8050498
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Fungi
  Other : JoF
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Basel : MDPI
Pages: - Volume / Issue: 8 (5) Sequence Number: 498 Start / End Page: - Identifier: ISSN: 2309-608X
CoNE: https://pure.mpg.de/cone/journals/resource/2309-608X