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  Sequestosome 1 is part of the interaction network of VAPB

James, C., Lenz, C., Urlaub, H., & Kehlenbach, R. H. (2021). Sequestosome 1 is part of the interaction network of VAPB. International Journal of Molecular Sciences, 22(24): 13271. doi:10.3390/ijms222413271.

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James, C., Author
Lenz, C.1, Author              
Urlaub, H.1, Author              
Kehlenbach, R H., Author
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1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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Free keywords: VAPB; amyotrophic lateral sclerosis; SQSTM1; sequestosome; p62
 Abstract: VAPB (Vesicle-Associated-membrane Protein-associated protein B) is a tail-anchored membrane protein of the endoplasmic reticulum that can also be detected at the inner nuclear membrane. As a component of many contact sites between the endoplasmic reticulum and other organelles, VAPB is engaged in multiple protein interactions with a plethora of binding partners. A mutant version of VAPB, P56S-VAPB, which results from a single point mutation, is involved in a familial form of amyotrophic lateral sclerosis (ALS8). We performed RAPIDS (rapamycin- and APEX-dependent identification of proteins by SILAC) to identify proteins that interact with or are in close proximity to P56S-VAPB. The mutation abrogates the interaction of VAPB with many known binding partners. Here, we identify Sequestosome 1 (SQSTM1), a well-known autophagic adapter protein, as a major interaction/proximity partner of P56S-VAPB. Remarkably, not only the mutant protein, but also wild-type VAPB interacts with SQSTM1, as shown by proximity ligation assays and co-immunoprecipiation experiments.

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Language(s): eng - English
 Dates: 2021-12-09
 Publication Status: Published online
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/ijms222413271
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Title: International Journal of Molecular Sciences
Source Genre: Journal
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Pages: 21 Volume / Issue: 22 (24) Sequence Number: 13271 Start / End Page: - Identifier: ISSN: 1422-0067