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  Method of platinum-carbon coating of ultrathin sections for transmission and scanning electron microscopy: An application for study of biological composites

Schwarz, H., & Gorb, S. (2003). Method of platinum-carbon coating of ultrathin sections for transmission and scanning electron microscopy: An application for study of biological composites. Microscopy Research and Technique, 62(3), 218-224. doi:10.1002/jemt.10343.

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 Creators:
Schwarz, H1, Author           
Gorb, S2, Author           
Affiliations:
1Electron Microscopy, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375794              
2Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society, Max-Planck-Ring 5, 72076 Tübingen, DE, ou_3375718              

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 Abstract: Many biological materials are composites containing two or more components with different mechanical properties. This study is concerned with the application of a method of platinum-carbon coating (Pt/C) of ultrathin sections for TEM and SEM studies of the design of natural composite materials. The changes in profile of the ultrathin resin-embedded sections during different stages of the preparation reflect the material properties of the various components: stiffer regions deform less than softer ones. Such changes in the section profile can be visualized by the Pt/C method and used as evidence of specific material properties in particular regions of composite materials. The method increases the relief contrast, improves the 3D-view of structures, and in combination with standard TEM and SEM procedures can provide clear demonstrations of material design. The distribution of chitin crystallites in the insect cuticle and the ultrastructure of the pore canal system specialized for the transport of epidermal secretions to the cuticle surface were studied here as examples.

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Language(s): eng - English
 Dates: 2003-10
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/jemt.10343
PMID: 14506687
 Degree: -

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Title: Microscopy Research and Technique
  Abbreviation : Microsc. Res. Tech.
Source Genre: Journal
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Publ. Info: New York, NY : Wiley-Liss
Pages: - Volume / Issue: 62 (3) Sequence Number: - Start / End Page: 218 - 224 Identifier: ISSN: 1059-910X
CoNE: https://pure.mpg.de/cone/journals/resource/954927686720