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  Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling

Barysch, S. V., Stankovic‐Valentin, N., Miedema, K.-T., Karaca, S., Doppel, J., Nait Achour, T., et al. (2021). Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling. EMBO Reports, 22(3): e49651. doi:10.15252/embr.201949651.

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Barysch, S. V., Author
Stankovic‐Valentin, N., Author
Miedema, K.-T., Author
Karaca, S.1, Author           
Doppel, J., Author
Nait Achour, T., Author
Vasudeva, A., Author
Wolf, L., Author
Sticht, C., Author
Urlaub, H.1, Author           
Melchior, F., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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Free keywords: ATF3; DUSP1; EGFR; IRF2BP1; SUMO
 Abstract: Molecular switches are essential modules in signaling networksand transcriptional reprogramming. Here, we describe a role forsmall ubiquitin-related modifier SUMO as a molecular switch inepidermal growth factor receptor (EGFR) signaling. Using quantita-tive mass spectrometry, we compare the endogenous SUMOproteomes of HeLa cells before and after EGF stimulation. Thereby,we identify a small group of transcriptional coregulators includingIRF2BP1, IRF2BP2, and IRF2BPL as novel players in EGFR signaling.Comparison of cells expressing wild type or SUMOylation-deficientIRF2BP1indicates that transient deSUMOylation of IRF2BP proteinsis important for appropriate expression of immediate early genesincludingdual specificity phosphatase1(DUSP1, MKP-1) and thetranscription factor ATF3. We find that IRF2BP1is a repressor,whose transient deSUMOylation on the DUSP1promoter allows—and whose timely reSUMOylation restricts—DUSP1transcription.Our work thus provides a paradigm how comparative SUMOproteome analyses serve to reveal novel regulators in signal trans-duction and transcription.

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Language(s): eng - English
 Dates: 2021-01-222021-03-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.15252/embr.201949651
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Title: EMBO Reports
Source Genre: Journal
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Pages: 16 Volume / Issue: 22 (3) Sequence Number: e49651 Start / End Page: - Identifier: ISSN: 1469-221X
ISSN: 1469-3178