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  Dextran as internal calibrant for N-glycan analysis by liquid chromatography coupled to ion mobility-mass spectrometry

Manz, C., Götze, M., Frank, C., Zappe, A., & Pagel, K. (2022). Dextran as internal calibrant for N-glycan analysis by liquid chromatography coupled to ion mobility-mass spectrometry. Analytical and Bioanalytical Chemistry, 414(17), 5023-5031. doi:10.1007/s00216-022-04133-0.

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Manz2022_Article_DextranAsInternalCalibrantForN.pdf (Publisher version), 2MB
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 Creators:
Manz, Christian1, 2, 3, Author           
Götze, Michael1, 2, Author
Frank, Clemens2, Author
Zappe, Andreas1, 2, Author
Pagel, Kevin1, 2, Author           
Affiliations:
1Molecular Physics, Fritz Haber Institute, Max Planck Society, ou_634545              
2Department of Chemistry and Biochemistry, Freie Universität Berlin, Altensteinstr. 23A, 14195 Berlin, Germany, ou_persistent22              
3Analytical Chemistry, CMC, Silence Therapeutics GmbH, Robert-Rössle-Str. 10, 13125 Berlin, Germany, ou_persistent22              

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Free keywords: N-Glycan analysis; HILIC; Ion mobility spectrometry; Calibration; Glucose units; Collision cross sections
 Abstract: LC–MS is one of the most important tools for the comprehensive characterization of N-glycans. Despite many efforts to speed up glycan analysis via optimized sample preparation (e.g., faster enzyme digestion in combination with instant or rapid labeling dyes), a major bottleneck remains the rather long measurement times of HILIC chromatography. Further compli- cation arises from the necessity to concomitantly calibrate with an external standard to allow for accurate retention times and the conversion into more robust GU values. Here we demonstrate the use of an internal calibration strategy for HILIC chromatography to speed up glycan analysis. By reducing the number of utilized dextran oligosaccharides, the calibrant can be spiked directly into the sample such that external calibration runs are no longer required. The minimized dextran ladder shows accurate GU calibration with a minor deviation of well below 1% and can be applied without modifications in sample preparation or data processing. We further demonstrate the simultaneous use of the minimized dextran ladder as calibrant for the estimation of CCS values in traveling wave ion mobility spectrometry. In both cases, the minimized dextran ladder enables the measurement of calibrant and sample in a single HPLC run without losing information or accuracy.

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Language(s): eng - English
 Dates: 2022-03-072022-05-162022-05-262022-07
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s00216-022-04133-0
 Degree: -

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Title: Analytical and Bioanalytical Chemistry
  Abbreviation : Anal. Bioanal. Chem.
Source Genre: Journal
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Publ. Info: Heidelberg : Springer-Verlag
Pages: 9 Volume / Issue: 414 (17) Sequence Number: - Start / End Page: 5023 - 5031 Identifier: ISSN: 1618-2642
CoNE: https://pure.mpg.de/cone/journals/resource/111006469468428