Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Unifying perspective of the ultrafast photodynamics of orange carotenoid proteins from synechocystis: peril of high-power excitation, existence of different S* states, and influence of tagging

Niziński, S., Wilson, A., Uriarte, L. M., Ruckebusch, C., Andreeva, E. A., Schlichting, I., et al. (2022). Unifying perspective of the ultrafast photodynamics of orange carotenoid proteins from synechocystis: peril of high-power excitation, existence of different S* states, and influence of tagging. JACS Au, 2(5), 1084-1095. doi:10.1021/jacsau.1c00472.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
JACSAu_2_2022_1084.pdf (beliebiger Volltext), 5MB
 
Datei-Permalink:
-
Name:
JACSAu_2_2022_1084.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Medical Research, MHMF; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
JACSAu_2_2022_1084_Suppl.pdf (Ergänzendes Material), 2MB
 
Datei-Permalink:
-
Name:
JACSAu_2_2022_1084_Suppl.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Medical Research, MHMF; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
https://pubs.acs.org/doi/10.1021/jacsau.1c00472 (beliebiger Volltext)
Beschreibung:
-
OA-Status:
externe Referenz:
https://pubs.acs.org/doi/pdf/10.1021/jacsau.1c00472 (beliebiger Volltext)
Beschreibung:
-
OA-Status:
Beschreibung:
-
OA-Status:

Urheber

einblenden:
ausblenden:
 Urheber:
Niziński, Stanisław, Autor
Wilson, Adjéle, Autor
Uriarte, Lucas M., Autor
Ruckebusch, Cyril, Autor
Andreeva, Elena A.1, Autor           
Schlichting, Ilme2, Autor           
Colletier, Jacques-Philippe, Autor
Kirilovsky, Diana, Autor
Burdzinski, Gotard, Autor
Sliwa, Michel, Autor
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Inhalt

einblenden:
ausblenden:
Schlagwörter: orange carotenoid protein, photodynamics, ultrafast spectroscopy, multiphoton absorption, cation radical, echinenone, quantum yield, His-tag
 Zusammenfassung: A substantial number of Orange Carotenoid Protein (OCP) studies have aimed to describe the evolution of singlet excited states leading to the formation of a photoactivated form, OCPR. The most recent one suggests that 3 ps-lived excited states are formed after the sub-100 fs decay of the initial S2 state. The S* state, which has the longest reported lifetime of a few to tens of picoseconds, is considered to be the precursor of the first red photoproduct P1. Here, we report the ultrafast photodynamics of the OCP from Synechocystis PCC 6803 carried out using visible–near infrared femtosecond time-resolved absorption spectroscopy as a function of the excitation pulse power and wavelength. We found that a carotenoid radical cation can form even at relatively low excitation power, obscuring the determination of photoactivation yields for P1. Moreover, the comparison of green (540 nm) and blue (470 nm) excitations revealed the existence of an hitherto uncharacterized excited state, denoted as S∼, living a few tens of picoseconds and formed only upon 470 nm excitation. Because neither the P1 quantum yield nor the photoactivation speed over hundreds of seconds vary under green and blue continuous irradiation, this S∼ species is unlikely to be involved in the photoactivation mechanism leading to OCPR. We also addressed the effect of His-tagging at the N- or C-termini on the excited-state photophysical properties. Differences in spectral signatures and lifetimes of the different excited states were observed at a variance with the usual assumption that His-tagging hardly influences protein dynamics and function. Altogether our results advocate for the careful consideration of the excitation power and His-tag position when comparing the photoactivation of different OCP variants and beg to revisit the notion that S* is the precursor of photoactivated OCPR.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2022-02-022021-10-222022-02-032022-04-252022-05-23
 Publikationsstatus: Erschienen
 Seiten: 12
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: JACS Au
  Kurztitel : JACS Au
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington, DC : American Chemical Society
Seiten: - Band / Heft: 2 (5) Artikelnummer: - Start- / Endseite: 1084 - 1095 Identifikator: ISSN: 2691-3704
CoNE: https://pure.mpg.de/cone/journals/resource/2691-3704