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  Isolation of naturally associated bacteria of necromenic Pristionchus nematodes and fitness consequences

Rae, R., Riebesell, M., Dinkelacker, I., Wang, Q., Herrmann, M., & Weller, A. (2008). Isolation of naturally associated bacteria of necromenic Pristionchus nematodes and fitness consequences. The Journal of Experimental Biology, 211(12), 1927-1936. doi:10.1242/jeb.014944.

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Rae, R1, Author           
Riebesell, M1, Author           
Dinkelacker, I1, Author           
Wang, Q1, Author           
Herrmann, M1, 2, Author           
Weller, AM1, Author           
Affiliations:
1Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375786              
2Entomo-Nematology Group, Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3507713              

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 Abstract: Nematodes and bacteria are major components of the soil ecosystem. Many nematodes use bacteria for food, whereas others evolved specialized bacterial interactions ranging from mutualism to parasitism. Little is known about the biological mechanisms by which nematode-bacterial interactions are achieved, largely because in the laboratory nematodes are often cultured under artificial conditions. We investigated the bacterial interactions of nematodes from the genus Pristionchus that have a strong association with scarab beetles. Pristionchus has a different feeding strategy than Caenorhabditis and meta-genomic 16S sequence analysis of Pristionchus individuals showed a diversity of living bacteria within the nematode gut and on the nematode cuticle. Twenty-three different bacterial strains were isolated from three Pristionchus-beetle associations and were used to study nematode-bacterial interactions under controlled laboratory conditions. We show a continuum of bacterial interactions from dissemination, to reduction in brood size and nematode mortality caused by bacteria derived from insect hosts. Olfactory discrimination experiments show distinct chemoattraction and fitness profiles of Pristionchus nematodes when exposed to different bacteria. For example, Pristionchus pacificus avoids Serratia marcescens possibly because of pathogenicity. Also, P. pacificus avoids Bacillus thuringiensis and insect pathogenic bacteria but is resistant to the human pathogens Staphylococcus aureus and Pseudomonas aeruginosa, unlike Caenorhabditis elegans. Pristionchus specifically recognize and respond to bacteria that cause ill health. Bringing the nematode-bacterial interaction into the laboratory allows detailed functional studies, including the genetic manipulation of the interaction in both nematodes and bacteria.

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 Dates: 2008-06
 Publication Status: Issued
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 Identifiers: DOI: 10.1242/jeb.014944
PMID: 18515723
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Title: The Journal of Experimental Biology
Source Genre: Journal
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Publ. Info: London : Published for the Company of Biologists Ltd. by the Cambridge University Press
Pages: - Volume / Issue: 211 (12) Sequence Number: - Start / End Page: 1927 - 1936 Identifier: ISSN: 0022-0949
CoNE: https://pure.mpg.de/cone/journals/resource/110992357319088_1