English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Live fluorescence imaging of F-actin organization in chick whole embryo cultures using SiR-actin

Schmitz-Elbers, M., Lukinavicius, G., & Smit, T. H. (2021). Live fluorescence imaging of F-actin organization in chick whole embryo cultures using SiR-actin. Cells, 10: 1578. doi:10.3390/cells10071578.

Item is

Files

show Files
hide Files
:
3386755.pdf (Publisher version), 2MB
Name:
3386755.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Schmitz-Elbers, M. , Author
Lukinavicius, G.1, Author           
Smit, T. H., Author
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

Content

show
hide
Free keywords: live fluorescence imaging; whole embryo culture; SiR-actin; F-actin
 Abstract: Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation and cytokinesis and mediate mechanical signaling. Here, we stain F-actin with the fluorogenic probe SiR-actin for live fluorescence imaging of whole chick embryos. We found that 50 nM SiR-actin in the culture medium is a safe and effective concentration for this purpose, as it provides high labeling density without inducing morphological malformations.

Details

show
hide
Language(s): eng - English
 Dates: 2021-06-22
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/cells10071578
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cells
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 10 Sequence Number: 1578 Start / End Page: - Identifier: ISSN: 2073-4409