English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Proteomic mapping of atrial and ventricular heart tissue in patients with aortic valve stenosis

Barbarics, B., Eildermann, K., Kaderali, L., Cyganek, L., Plessmann, U., Bodemeyer, J., et al. (2021). Proteomic mapping of atrial and ventricular heart tissue in patients with aortic valve stenosis. Scientific Reports, 11: 24389. doi:10.1038/s41598-021-03907-3.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
3387151.pdf (Publisher version), 3MB
Name:
3387151.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Barbarics, B., Author
Eildermann, K., Author
Kaderali, L., Author
Cyganek, L., Author
Plessmann, U.1, Author           
Bodemeyer, J., Author
Paul, T., Author
Ströbel, P., Author
Urlaub, H.1, Author           
Tirilomis, T., Author
Lenz, C.1, Author           
Bohnenberger, H., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

Content

show
hide
Free keywords: -
 Abstract: Aortic valve stenosis (AVS) is one of the most common valve diseases in the world. However, detailed biological understanding of the myocardial changes in AVS hearts on the proteome level is still lacking. Proteomic studies using high-resolution mass spectrometry of formalin-fixed and paraffin-embedded (FFPE) human myocardial tissue of AVS-patients are very rare due to methodical issues. To overcome these issues this study used high resolution mass spectrometry in combination with a stem cell- derived cardiac specific protein quantification-standard to profile the proteomes of 17 atrial and 29 left ventricular myocardial FFPE human myocardial tissue samples from AVS-patients. In our proteomic analysis we quantified a median of 1980 (range 1495–2281) proteins in every single sample and identified significant upregulation of 239 proteins in atrial and 54 proteins in ventricular myocardium. We compared the proteins with published data. Well studied proteins reflect disease-related changes in AVS, such as cardiac hypertrophy, development of fibrosis, impairment of mitochondria and downregulated blood supply. In summary, we provide both a workflow for quantitative proteomics of human FFPE heart tissue and a comprehensive proteomic resource for AVS induced changes in the human myocardium.

Details

show
hide
Language(s): eng - English
 Dates: 2021-12-22
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41598-021-03907-3
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Scientific Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 12 Volume / Issue: 11 Sequence Number: 24389 Start / End Page: - Identifier: ISSN: 2045-2322