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  GM1 asymmetry in the membrane stabilizes pores

Aleksanyan, M., Lira, R. d., Steinkühler, J., & Dimova, R. (2022). GM1 asymmetry in the membrane stabilizes pores. Biophysical Journal. doi:10.1016/j.bpj.2022.06.011.

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Aleksanyan, Mina1, Author              
Lira, Rafael de1, Author              
Steinkühler, Jan1, Author              
Dimova, Rumiana1, Author              
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1Rumiana Dimova, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_3360040              

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 Abstract: Cell membranes are highly asymmetric and their stability against poration is crucial for survival. We investigated the influence of membrane asymmetry on electroporation of giant unilamellar vesicles with membranes doped with GM1, a ganglioside asymmetrically enriched in the outer leaflet of neuronal cell membranes. Compared to symmetric membranes, the lifetimes of micronsized pores are about an order of magnitude longer suggesting that pores are stabilized by GM1. Internal membrane nanotubes caused by the GM1 asymmetry, obstruct and additionally slow down pore closure, effectively reducing pore edge tension and leading to leaky membranes. Our results point to the drastic effects this ganglioside can have on pore resealing in biotechnology applications based on poration as well as on membrane repair processes.

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Language(s): eng - English
 Dates: 2022-06-06
 Publication Status: Published online
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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0006-3495

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Title: bioRxiv : the preprint server for biology
Source Genre: Journal
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Publ. Info: Cold Spring Harbor : Cold Spring Haroor Laboratory
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ZDB: 2766415-6