English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Bottom-up assembly of target-specific cytotoxic synthetic cells

Hernandez Bücher, J. E., Staufer, O., Ostertag, L., Mersdorf, U., Platzman, I., & Spatz, J. P. (2022). Bottom-up assembly of target-specific cytotoxic synthetic cells. Biomaterials, 285: 121522, pp. 1-13. doi:10.1016/j.biomaterials.2022.121522.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Biomaterials_285_2022_121522.pdf (Any fulltext), 6MB
 
File Permalink:
-
Name:
Biomaterials_285_2022_121522.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
Biomaterials_285_2022_121522_Suppl.zip (Supplementary material), 19MB
 
File Permalink:
-
Name:
Biomaterials_285_2022_121522_Suppl.zip
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/zip
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Hernandez Bücher, Jochen Estebano1, Author              
Staufer, Oskar1, Author              
Ostertag, Lukas1, Author              
Mersdorf, Ulrike2, Author              
Platzman, Ilia1, Author              
Spatz, Joachim Pius1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Content

show
hide
Free keywords: Giant unilamellar vesicles; FasL; Bottom-up synthetic biology; Immunotherapy; Immunological synapse
 Abstract: Immune vigilance ensures body integrity by eliminating malignant cells through the complex but coordinated cooperation of highly diversified lymphocytes populations. The sheer complexity of the immune system has slowed development of immunotherapies based on top-down genetic engineering of lymphocytes. In contrast, bottom-up assembly of synthetic cell compartments has contributed novel engineering strategies to reverse engineer and understand cellular phenomena as molecularly defined systems. Towards reducing the complexity of immunological systems, herein, a bottom-up approach for controlled assembly of fully-synthetic immune-inspired cells from predefined molecular components based on giant unilamellar vesicles is described. For construction of target-specific cytotoxic immune cells, the Fas-ligand-based apoptosis-inducing immune cell module is combined with an antibody-mediated cellular cytotoxicity-inspired system. The designed immune cells identify leukemia cells by specific surface antigens. Subsequently, they form stable attachments sites and eliminate their targets by induction of apoptosis. A structural and functional characterization of the synthetic immune cells by means of microfluidics, live cell, confocal and electron microscopy, dynamic light scattering as well as flow cytometry is presented. This study demonstrates the bioinspired construction of effector immune cells from defined molecular building blocks, enabling learning-by-building approaches in synthetic immunology.

Details

show
hide
Language(s): eng - English
 Dates: 2022-03-312021-09-212022-04-102022-04-132022-06
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biomaterials
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Guildford, England : Elsevier
Pages: - Volume / Issue: 285 Sequence Number: 121522 Start / End Page: 1 - 13 Identifier: ISSN: 0142-9612
CoNE: https://pure.mpg.de/cone/journals/resource/954925472369