English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates

Nakamaye, K. L., Gish, G., Eckstein, F., & Vosberg, H.-P. (1988). Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates. Nucleic Acids Research (London), 16(21), 9947-9959. doi:10.1093/nar/16.21.9947.

Item is

Files

show Files
hide Files
:
NuclAcidRes_16_1988_9947.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
NuclAcidRes_16_1988_9947.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
OA-Status:
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Nakamaye, Kay L., Author
Gish, Gerald, Author
Eckstein, Fritz, Author
Vosberg, Hans-Peter1, Author           
Affiliations:
1Department of Molecular Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497702              

Content

show
hide
Free keywords: -
 Abstract: The direct sequencing of DNA generated by the polynucleotide chain reaction, via the incorporation of phosphorothioate nucleotides and followed by treatment with an alkylating reagent that cleaves specifically at the phosphorothioate positions, is described. The Taq polymerase used in the amplification reaction incorporates the Sp-diastereomer of the deoxynucleoside 5'-O-(1-thiotriphosphates) as efficiently as the natural nucleotides. Chemical degradation of the phosphorothioate-containing DNA fragment can be performed with either 2-iodoethanol or 2,3-epoxy-1-propanol. The higher reactivity of 2,3-epoxy-1-propanol allows less reagent to be used to obtain the same amount of degradation as with 2-iodoethanol.

Details

show
hide
Language(s): eng - English
 Dates: 1988-09-051988-10-071988-11-11
 Publication Status: Issued
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nucleic Acids Research (London)
  Other : Nucleic Acids Res
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford : Oxford University Press
Pages: - Volume / Issue: 16 (21) Sequence Number: - Start / End Page: 9947 - 9959 Identifier: ISSN: 0305-1048
CoNE: https://pure.mpg.de/cone/journals/resource/110992357379342