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  Autophagy modulates the metabolism and growth of tomato fruit during development

Alseekh, S., Zhu, F., Vallarino, J. G., Sokolowska, E., Yoshida, T., Bergmann, S., et al. (2022). Autophagy modulates the metabolism and growth of tomato fruit during development. Horticulture Research, uhac129. doi:10.1093/hr/uhac129.

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 Creators:
Alseekh, S.1, Author              
Zhu, F.2, Author              
Vallarino, J. G.2, Author              
Sokolowska, E.3, Author              
Yoshida, T.2, Author              
Bergmann, S.2, Author              
Wendenburg, R.1, Author              
Bolze, A.2, Author              
Skirycz, A.3, Author              
Avin-Wittenberg, T.2, Author              
Fernie, A. R.2, Author              
Affiliations:
1The Genetics of Crop Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_3244836              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
3Small-Molecule Signalling, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2586692              

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 Abstract: Although autophagy is a conserved mechanism operating across eukaryotes, its effects on crops and especially their metabolism has received relatively little attention. Indeed, whilst a few recent studies have used systems biology tools to look at the consequences of lack of autophagy in maize these focused on leaf tissues rather than the kernels. Here we utilized RNA interference (RNAi) to generate tomato plants that were deficient in the autophagy-regulating protease ATG4. Plants displayed an early senescence phenotype yet relatively mild changes in the foliar metabolome and were characterized by a reduced fruit yield phenotype. Metabolite profiling indicated that metabolites of ATG4-RNAi tomato leaves just exhibited minor alterations while that of fruit displayed bigger difference compared to the WT. In detail, many primary metabolites exhibited decreases in the ATG4-RNAi lines, such as proline, tryptophan and phenylalanine, while the representative secondary metabolites (quinic acid and 3-trans-caffeoylquinic acid) were present at substantially higher levels in ATG4-RNAi green fruits than in WT. Moreover, transcriptome analysis indicated that the most prominent differences were in the significant upregulation of organelle degradation genes involved in the proteasome or chloroplast vesiculation pathways, which was further confirmed by the reduced levels of chloroplastic proteins in the proteomics data. Furthermore, integration analysis of the metabolome, transcriptome and proteome data indicated that ATG4 significantly affected the lipid metabolism, chlorophyll binding proteins and chloroplast biosynthesis. These data collectively lead us to propose a more sophisticated model to explain the cellular co-ordination of the process of autophagy.

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Language(s): eng - English
 Dates: 2022-06-132022-06
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.1093/hr/uhac129
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Title: Horticulture Research
  Alternative Title : Hortic Res
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: uhac129 Start / End Page: - Identifier: ISBN: 2052-7276