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  Matrix-trapped viruses can prevent invasion of bacterial biofilms by colonizing cells

Bond, M. C., Vidakovic, L., Singh, P. K., Drescher, K., & Nadell, C. D. (2021). Matrix-trapped viruses can prevent invasion of bacterial biofilms by colonizing cells. eLife, 10: e65355. doi:10.7554/eLife.65355.

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https://doi.org/10.7554/eLife.65355 (Publisher version)
Description:
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OA-Status:
Gold

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 Creators:
Bond, Matthew C., Author
Vidakovic, Lucia1, Author           
Singh, Praveen K.1, Author           
Drescher, Knut1, 2, 3, Author           
Nadell, Carey D., Author
Affiliations:
1Max Planck Research Group Bacterial Biofilms, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266298              
2Biozentrum, University of Basel, Basel, Switzerland, ou_persistent22              
3Department of Physics, Philipps University Marburg, Marburg, Germany, ou_persistent22              

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Free keywords: Bacteria/*growth & development/*virology Bacterial Physiological Phenomena Bacteriophages/*physiology *Biofilms/growth & development Escherichia coli/physiology/virology *E. coli *bacteriophage *biofilm *community assembly *ecology *extracellular matrix *invasion *spatial ecology
 Abstract: Bacteriophages can be trapped in the matrix of bacterial biofilms, such that the cells inside them are protected. It is not known whether these phages are still infectious and whether they pose a threat to newly arriving bacteria. Here, we address these questions using Escherichia coli and its lytic phage T7. Prior work has demonstrated that T7 phages are bound in the outermost curli polymer layers of the E. coli biofilm matrix. We show that these phages do remain viable and can kill colonizing cells that are T7-susceptible. If cells colonize a resident biofilm before phages do, we find that they can still be killed by phage exposure if it occurs soon thereafter. However, if colonizing cells are present on the biofilm long enough before phage exposure, they gain phage protection via envelopment within curli-producing clusters of the resident biofilm cells.

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Language(s): eng - English
 Dates: 2021-07-10
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: Other: 34240700
DOI: 10.7554/eLife.65355
ISSN: 2050-084x
 Degree: -

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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 10 Sequence Number: e65355 Start / End Page: - Identifier: Other: URL
ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X