Fast native-SAD phasing for routine macromolecular structure determination

Weinert, T; Olieric, V; Waltersperger, S; Panepucci, E; Chen, L; Zhang, H; Zhou, D; Rose, J; Ebihara, A; Kuramitsu, S; Li, D; Howe, N; Schnapp, G; Pautsch, A; Bargsten, K; Prota, AE; Surana, P; Kottur, J; Jair, DT; Basilico, F; Cecatiello, V; Pasqualato, S; Boland, A; Weichenrieder, O; Wang, B-C; Steinmetz, MO; Caffrey, M; Wang, M

doi:10.1038/nmeth.3211

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Fast native-SAD phasing for routine macromolecular structure determination

Weinert, T., Olieric, V., Waltersperger, S., Panepucci, E., Chen, L., Zhang, H., et al. (2015). Fast native-SAD phasing for routine macromolecular structure determination. Nature Methods, 12(2), 131-133. doi:10.1038/nmeth.3211.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-A4F0-D Version Permalink: https://hdl.handle.net/21.11116/0000-000C-8FC5-5

Genre: Journal Article

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Weinert, T, Author
Olieric, V, Author
Waltersperger, S, Author
Panepucci, E, Author
Chen, L, Author
Zhang, H, Author
Zhou, D, Author
Rose, J, Author
Ebihara, A, Author
Kuramitsu, S, Author
Li, D, Author
Howe, N, Author
Schnapp, G, Author
Pautsch, A, Author
Bargsten, K, Author
Prota, AE, Author
Surana, P, Author
Kottur, J, Author
Jair, DT, Author
Basilico, F, Author
Cecatiello, V, AuthorPasqualato, S, AuthorBoland, A^{1, 2}, Author Weichenrieder, O^{1, 2}, Author Wang, B-C, AuthorSteinmetz, MO, AuthorCaffrey, M, AuthorWang, M, Author more..

Affiliations:
1Structural Biology of Selfish RNA, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3490031
2Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society, Max-Planck-Ring 5, 72076 Tübingen, DE, ou_3375718

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Abstract: We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

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Dates: Date issued: 2015-02

Publication Status: Issued

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Identifiers: DOI: 10.1038/nmeth.3211
PMID: 25506719

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Title: Nature Methods

Other : Nature Methods

Source Genre: Journal

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Publ. Info: New York, NY : Nature Publishing Group

Pages: - Volume / Issue: 12 (2) Sequence Number: - Start / End Page: 131 - 133 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556