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  Coumarin-phalloidin: a new actin probe permitting triple immunofluorescence microscopy of the cytoskeleton

Small, J., Zobeley, S., Rinnerthaler, G., & Faulstich, H. (1988). Coumarin-phalloidin: a new actin probe permitting triple immunofluorescence microscopy of the cytoskeleton. Journal of Cell Science, 89, 21-24. doi:10.1242/jcs.89.1.21.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-A6DB-4 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-A6DC-3
Genre: Journal Article

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JCellSci_89_1988_21.pdf (Any fulltext), 2MB
 
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 Creators:
Small, J.V., Author
Zobeley, S.1, Author           
Rinnerthaler, G., Author
Faulstich, H.1, Author           
Affiliations:
1Department of Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_3321127              

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Free keywords: coumarin-phalloidin, actin, immunofluorescence microscopy
 Abstract: 7-Diethylamino-3-(4-isothiocyanotophenyl)-4-methylcoumarin (CPITC) was coupled to amino-methyldithiolanophalloidin to produce a new phalloidin derivative, coumarin-phalloidin, fluorescent in the blue region of the spectrum. Coumarin-phalloidin binds to actin with around 100-fold less affinity than unconjugated phalloidin, but with enough avidity to make it a useful stain for actin filaments. Appropriate filter combinations permit triple immunofluorescence microscopy of the cytoskeleton with fluorescein and rhodamine conjugates together with coumarin-phalloidin.

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Language(s): eng - English
 Dates: 1987-09-141987-10-021988-01
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://pubmed.ncbi.nlm.nih.gov/2458368/
DOI: 10.1242/jcs.89.1.21
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Title: Journal of Cell Science
Source Genre: Journal
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Publ. Info: Cambridge, U.K. : Co. of Biologists
Pages: - Volume / Issue: 89 Sequence Number: - Start / End Page: 21 - 24 Identifier: ISSN: 0021-9533
CoNE: https://pure.mpg.de/cone/journals/resource/954925326678