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  Structural insights into photosystem II assembly

Zabret, J., Bohn, S., Schuller, S. K., Arnolds, O., Moller, M., Meier-Credo, J., et al. (2021). Structural insights into photosystem II assembly. Nature Plants, 7(4), 524-538. doi:10.1038/s41477-021-00895-0.

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 Creators:
Zabret, Jure1, Author
Bohn, Stefan2, Author           
Schuller, Sandra K.3, Author           
Arnolds, Oliver1, Author
Moller, Madeline1, Author
Meier-Credo, Jakob1, Author
Liauw, Pasqual1, Author
Chan, Aaron1, Author
Tajkhorshid, Emad1, Author
Langer, Julian D.1, Author
Stoll, Raphael1, Author
Krieger-Liszkay, Anja1, Author
Engel, Benjamin D.2, Author           
Rudack, Till1, Author
Schuller, Jan M.2, Author           
Nowaczyk, Marc M.1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
3Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              

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Free keywords: Plant Sciences;
 Abstract: Biogenesis of photosystem II (PSII), nature's water-splitting catalyst, is assisted by auxiliary proteins that form transient complexes with PSII components to facilitate stepwise assembly events. Using cryo-electron microscopy, we solved the structure of such a PSII assembly intermediate from Thermosynechococcus elongatus at 2.94 angstrom resolution. It contains three assembly factors (Psb27, Psb28 and Psb34) and provides detailed insights into their molecular function. Binding of Psb28 induces large conformational changes at the PSII acceptor side, which distort the binding pocket of the mobile quinone (Q(B)) and replace the bicarbonate ligand of non-haem iron with glutamate, a structural motif found in reaction centres of non-oxygenic photosynthetic bacteria. These results reveal mechanisms that protect PSII from damage during biogenesis until water splitting is activated. Our structure further demonstrates how the PSII active site is prepared for the incorporation of the Mn4CaO5 cluster, which performs the unique water-splitting reaction.
Photosystems need auxiliary proteins to assist their assembly. Cryo-electron microscopy of a cyanobacterial photosystem II assembly intermediate at 2.94 angstrom reveals mechanisms protecting against photodamage during vulnerable stages of biogenesis.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Nature Plants
Source Genre: Journal
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Publ. Info: HEIDELBERGER PLATZ 3, BERLIN, 14197, GERMANY : NATURE RESEARCH
Pages: - Volume / Issue: 7 (4) Sequence Number: - Start / End Page: 524 - 538 Identifier: ISSN: 2055-026X