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  Circadian life without micronutrients: effects of altered micronutrient supply on clock function in Arabidopsis

Salomé, P., Bernal, M., & Krämer, U. (2014). Circadian life without micronutrients: effects of altered micronutrient supply on clock function in Arabidopsis. In Methods in Molecular Biology. US: Springer.

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 Urheber:
Salomé, PA1, Autor           
Bernal, M, Autor
Krämer, U, Autor
Affiliations:
1Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375790              

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 Zusammenfassung: The plant circadian clock is formed by a number of interlocked feedback loops that control the expression of thousands of genes. Genetic and pharmacological approaches towards the study of the plant clock are routinely carried out on Murashige and Skoog growth medium, which is both Fe-replete and Cu-deficient. However, it has recently become clear that the plant clock responds to available iron (Fe) supply: circadian pace slows down under conditions of Fe deficiency; circadian period progressively shortens with increasing Fe supply. Here, we describe several growth media that may be used to study the effects of varying micronutrient supply on the circadian clock, in which deficiency in a given micronutrient are imposed by the addition of a specific chelator or, alternatively, by using EDTA-washed agar as gelling agent, thus minimizing micronutrient contamination.

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 Datum: 2014
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1007/978-1-4939-0700-7_16
PMID: 24792056
 Art des Abschluß: -

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Titel: Methods in Molecular Biology
  Andere : Methods Mol. Biol.
Genre der Quelle: Reihe
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: US : Springer
Seiten: - Band / Heft: 1158 Artikelnummer: - Start- / Endseite: - Identifikator: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544

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Titel: Plant Circadian Networks: Methods and Protocols
Genre der Quelle: Buch
 Urheber:
Staiger, D, Herausgeber
Affiliations:
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Ort, Verlag, Ausgabe: New York, NY, USA : Humana Press
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: 227 - 238 Identifikator: ISBN: 978-1-4939-0699-4