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Free keywords:
autocatalysis
in vitro systems
origin of life
self-replication
synthetic biology
in-vitro amplification
rna-polymerase
DNA-polymerase
ribosomal-rna
primer extension
autocatalytic synthesis
intervening sequence
structurally complex
nucleic-acids
splicing rna
Materials Science
Abstract:
A key characteristic of living systems is the storage and replication of information, and as such the development of self-replicating systems capable of heredity is of great importance to the fields of synthetic biology and origin of life research. In this review, the design and implementation of self-replicating systems in the context of bottom-up synthetic biology is discussed, with a particular focus on nucleic acid-based replication including nonenzymatic systems, ribozyme-based systems, and complex in vitro translation coupled RNA and DNA replication. The current state and remaining challenges of the respective fields are discussed, and the potential of individual replicators for synthetic biology applications such as the creation of artificial life capable of Darwinian evolution is also summarized.
