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  Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers

Schönberger, K., Mitterer, M., Büscher, J. M., & Cabezas-Wallscheid, N. (2022). Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers. STAR Protocols, 3: 101408. doi:10.1016/j.xpro.2022.101408.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-AB98-A Version Permalink: https://hdl.handle.net/21.11116/0000-000A-AB99-9
Genre: Journal Article

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10.1016_j.xpro.2022.101408.pdf (Publisher version), 4MB
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10.1016_j.xpro.2022.101408.pdf
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2022
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https://www.sciencedirect.com/science/article/pii/S266616672200288X (Publisher version)
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 Creators:
Schönberger, Katharina1, Author
Mitterer, Michael2, Author
Büscher, Jörg Martin2, Author           
Cabezas-Wallscheid, Nina1, Author           
Affiliations:
1Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243648              

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Free keywords: Metabolomics, Stem Cells, Mass Spectrometry
 Abstract: Metabolism is important for the regulation of hematopoietic stem cells (HSCs) and drives cellular fate. Due to the scarcity of HSCs, it has been technically challenging to perform metabolome analyses gaining insight into HSC metabolic regulatory networks. Here, we present two targeted liquid chromatography–mass spectrometry approaches that enable the detection of metabolites after fluorescence-activated cell sorting when sample amounts are limited. One protocol covers signaling lipids and retinoids, while the second detects tricarboxylic acid cycle metabolites and amino acids.

For complete details on the use and execution of this protocol, please refer to Schönberger et al. (2022).

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Language(s): eng - English
 Dates: 2022-06-17
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.xpro.2022.101408
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Title: STAR Protocols
Source Genre: Journal
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Publ. Info: Cambridge, MA ; Amsterdam : Cell Press ; Elsevier
Pages: - Volume / Issue: 3 Sequence Number: 101408 Start / End Page: - Identifier: ISSN: 2666-1667
CoNE: https://pure.mpg.de/cone/journals/resource/2666-1667