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  Bone material characteristics influenced by osteocytes

Kerschnitzki, M. (2012). Bone material characteristics influenced by osteocytes. PhD Thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I.

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 Creators:
Kerschnitzki, Michael1, Author           
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1Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863285              

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Free keywords: Bone formation; osteocyte networks; osteocytic osteolysis; small angle x-ray scattering (SAXS); confocal laser scanning microscopy (CLSM)
 Abstract: This thesis aims to test the hypothesis whether osteocytes have a direct influence on bone material properties in their vicinity. In this regard, the concomitant ana-lysis of osteocyte network organization and bone ultrastructural properties on the submicron level is the central approach to answer this question. In this work, it is shown that already initial cell-cell alignment during the process of bone forma-tion is crucial for the synthesis of highly organized bone. Furthermore it is pro-posed that the occurrence of highly ordered osteocyte networks visualized with confocal laser scanning microscopy (CLSM) has a strong impact on the ability of osteocytes to directly influence bone material properties. These highly organized networks are another consequence of initial cell-cell alignment and are found to be arranged such as to feature short mineral cell distances. Examination of sub-micron mineral properties with scanning small angle x-ray scattering (sSAXS) shows that bone material in the direct vicinity of osteocytes and their cell proc-esses shows different mineral properties compared to bone further away in the depth of the tissue. Moreover, mechanisms of passive mineral extraction from the mineralized surface of the osteocyte network, due to the treatment with calcium poor ionic solutions, are investigated. It is shown that this chemical process oc-curring under physiological conditions leads not only to the dissolution of con-siderable amounts of calcium, but also to efficient diffusion of these ions through the osteocyte network structures. Finally, medullary bone which is intended as a labile calcium source for daily egg shell formation in hens is used as a model system for rapid bone turnover rates. This bone type in particular indicates the importance of uniquely adapted, less stable mineral structures to fit the require-ments for rapid bone resorption as well as re-formation.

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Language(s): eng - English
 Dates: 2012
 Publication Status: Accepted / In Press
 Pages: 123 S.
 Publishing info: Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.18452/16479
 Degree: PhD

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