English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  C6orf203 is an RNA-binding protein involved in mitochondrial protein synthesis

Gopalakrishna, S., Pearce, S. F., Dinan, A. M., Schober, F. A., Cipullo, M., Spahr, H., et al. (2019). C6orf203 is an RNA-binding protein involved in mitochondrial protein synthesis. Nucleic Acids Res, 47(17), 9386-9399. doi:10.1093/nar/gkz684.

Item is

Files

show Files

Locators

show
hide
Description:
-
OA-Status:
Not specified

Creators

show
hide
 Creators:
Gopalakrishna, S.1, Author           
Pearce, S. F.1, Author           
Dinan, A. M., Author
Schober, F. A.2, Author           
Cipullo, M.1, Author           
Spahr, H2, Author           
Khawaja, A.1, Author           
Maffezzini, C.1, Author           
Freyer, C.2, Author           
Wredenberg, A.2, Author           
Atanassov, I.3, Author           
Firth, A. E., Author
Rorbach, J.1, Author           
Affiliations:
1Rorbach – Mitochondrial Gene Expression, External and Associated Groups, Max Planck Institute for Biology of Ageing, Max Planck Society, ou_3394012              
2Department Larsson - Mitochondrial Biology, Max Planck Institute for Biology of Ageing, Max Planck Society, ou_1942286              
3Proteomics, Core Facilities, Max Planck Institute for Biology of Ageing, Max Planck Society, ou_1942305              

Content

show
hide
Free keywords: Animals HEK293 Cells Humans Mitochondria/*genetics Mitochondrial Proteins/*biosynthesis/genetics/physiology Mitochondrial Ribosomes/metabolism RNA, Messenger/genetics RNA, Mitochondrial/*genetics RNA, Ribosomal/genetics RNA-Binding Proteins/*genetics/physiology
 Abstract: In all biological systems, RNAs are associated with RNA-binding proteins (RBPs), forming complexes that control gene regulatory mechanisms, from RNA synthesis to decay. In mammalian mitochondria, post-transcriptional regulation of gene expression is conducted by mitochondrial RBPs (mt-RBPs) at various stages of mt-RNA metabolism, including polycistronic transcript production, its processing into individual transcripts, mt-RNA modifications, stability, translation and degradation. To date, only a handful of mt-RBPs have been characterized. Here, we describe a putative human mitochondrial protein, C6orf203, that contains an S4-like domain-an evolutionarily conserved RNA-binding domain previously identified in proteins involved in translation. Our data show C6orf203 to bind highly structured RNA in vitro and associate with the mitoribosomal large subunit in HEK293T cells. Knockout of C6orf203 leads to a decrease in mitochondrial translation and consequent OXPHOS deficiency, without affecting mitochondrial RNA levels. Although mitoribosome stability is not affected in C6orf203-depleted cells, mitoribosome profiling analysis revealed a global disruption of the association of mt-mRNAs with the mitoribosome, suggesting that C6orf203 may be required for the proper maturation and functioning of the mitoribosome. We therefore propose C6orf203 to be a novel RNA-binding protein involved in mitochondrial translation, expanding the repertoire of factors engaged in this process.

Details

show
hide
Language(s):
 Dates: 2019-08-102019-08-10
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: 31396629
DOI: 10.1093/nar/gkz684
ISSN: 1362-4962 (Electronic)0305-1048 (Linking)
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nucleic Acids Res
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 47 (17) Sequence Number: - Start / End Page: 9386 - 9399 Identifier: -