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キーワード:
ATPases Associated with Diverse Cellular Activities/genetics/*metabolism
Gene Knockdown Techniques
HEK293 Cells
Humans
Membrane Proteins/genetics/*metabolism
Membrane Transport Proteins/genetics/metabolism
Metalloendopeptidases/genetics/*metabolism
Mitochondrial Membranes/*metabolism
Mitochondrial Precursor Protein Import Complex Proteins
Mitochondrial Proteins/genetics/*metabolism
Protein Transport/physiology
Saccharomyces cerevisiae
要旨:
The mitochondrial presequence translocation machinery (TIM23 complex) is conserved between the yeast Saccharomyces cerevisiae and humans; however, functional characterization has been mainly performed in yeast. Here, we define the constituents of the human TIM23 complex using mass spectrometry and identified ROMO1 as a new translocase constituent with an exceptionally short half-life. Analyses of a ROMO1 knockout cell line revealed aberrant inner membrane structure and altered processing of the GTPase OPA1. We show that in the absence of ROMO1, mitochondria lose the inner membrane YME1L protease, which participates in OPA1 processing and ROMO1 turnover. While ROMO1 is dispensable for general protein import along the presequence pathway, we show that it participates in the dynamics of TIM21 during respiratory chain biogenesis and is specifically required for import of YME1L. This selective import defect can be linked to charge distribution in the unusually long targeting sequence of YME1L. Our analyses establish an unexpected link between mitochondrial protein import and inner membrane protein quality control.
