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  Suppression of coatomer mutants by a new protein family with COPI and COPII binding motifs in Saccharomyces cerevisiae

Sandmann, T., Herrmann, J., Dengjel, J., Schwarz, H., & Spang, A. (2003). Suppression of coatomer mutants by a new protein family with COPI and COPII binding motifs in Saccharomyces cerevisiae. Molecular Biology of the Cell, 14(8), 3097-3113. doi:10.1091/mbc.e02-11-0736.

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Sandmann, T1, Author           
Herrmann, JM, Author
Dengjel, J, Author
Schwarz, H, Author           
Spang, A1, Author           
Affiliations:
1Spang Group, Friedrich Miescher Laboratory, Max Planck Society, ou_3393808              

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 Abstract: Protein trafficking is achieved by a bidirectional vesicle flow between the various compartments of the eukaryotic cell. COPII coated vesicles mediate anterograde protein transport from the endoplasmic reticulum to the Golgi apparatus, whereas retrograde Golgi-to-endoplasmic reticulum vesicles use the COPI coat. Inactivation of COPI vesicle formation in conditional sec21 (gamma-COP) mutants rapidly blocks transport of certain proteins along the early secretory pathway. We have identified the integral membrane protein Mst27p as a strong suppressor of sec21-3 and ret1-1 mutants. A C-terminal KKXX motif of Mst27p that allows direct binding to the COPI complex is crucial for its suppression ability. Mst27p and its homolog Yar033w (Mst28p) are part of the same complex. Both proteins contain cytoplasmic exposed C termini that have the ability to interact directly with COPI and COPII coat complexes. Site-specific mutations of the COPI binding domain abolished suppression of the sec21 mutants. Our results indicate that overexpression of MST27 provides an increased number of coat binding sites on membranes of the early secretory pathway and thereby promotes vesicle formation. As a consequence, the amount of cargo that can bind COPI might be important for the regulation of the vesicle flow in the early secretory pathway.

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 Dates: 2003-08
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.1091/mbc.e02-11-0736
PMID: 12925749
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Title: Molecular Biology of the Cell
  Other : Mol. Biol. Cell
Source Genre: Journal
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Publ. Info: American Society for Cell Biology
Pages: - Volume / Issue: 14 (8) Sequence Number: - Start / End Page: 3097 - 3113 Identifier: ISSN: 1059-1524
CoNE: https://pure.mpg.de/cone/journals/resource/954927716372_1