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  Multi-Modal Imaging of Corpora Amylacea in Post Mortem Human Midbrain

Lee, J., Mack, A., Longo, R., Tromba, G., Shiozawa, T., Scheffler, K., et al. (2022). Multi-Modal Imaging of Corpora Amylacea in Post Mortem Human Midbrain. Poster presented at FENS Forum 2022, Paris, France.

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Lee, JY1, Author           
Mack, A, Author
Longo, R, Author
Tromba, G, Author
Shiozawa, T, Author
Scheffler, K1, Author           
Hagberg, G1, Author           
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1Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497796              

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 Abstract: Aims Corpora amylacea, which contains polysaccharides, accumulates in human brain throughout aging. We aimed to use multi-modal imaging to locate corpora amylacea and investigate their three-dimensional morphological features. Methods Post mortem human brain stem samples (N=4) were scanned using high field MRI (14.1T, T2*weighted 62μm voxels) and Xray synchrotron-radiation-based phase-contrast microtomography (PhC-μCT, 1μm voxels). Using an automated segmentation pipeline, corpora amylacea were identified on PhC-μCT images and the diameters was calculated. Finally, the samples were sectioned and stained using Klüver-Barrera and Periodic-acid Schiff reaction. Results In both MRI and PhC- μCT, corpora amylacea rich regions showed signal contrast. Using automated segmentation of the PhC-μCT dataset, we detected over 40,000 corpora amylacea located at the intercollicular region that showed hyperintensity. In T2* weighted MRI, corpora amylacea rich regions had low signal due to T2* shortening. The median diameter of corpora amylacea was 12 μm. Sections stained with Periodic-acid Schiff reaction confirmed that these granular bodies are indeed corpora amylacea. Conclusions Corpora amylacea rich region shows noticeable contrast in T2* weighted MRI. In high resolution phase-contrast microtomography, individual corpora amylacea can be extracted.

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 Dates: 2022-07
 Publication Status: Published online
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Title: FENS Forum 2022
Place of Event: Paris, France
Start-/End Date: 2022-07-09 - 2022-07-13

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Title: FENS Forum 2022
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: S05-563 Start / End Page: 4760 Identifier: -