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  DNA base editing in nuclear and organellar genomes

Tan, J., Forner, J., Karcher, D., & Bock, R. (2022). DNA base editing in nuclear and organellar genomes. Trends in Genetics. doi:10.1016/j.tig.2022.06.015.

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Genre: Journal Article

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 Creators:
Tan, Junjie1, Author
Forner, J.2, Author           
Karcher, D.2, Author           
Bock, R.2, Author           
Affiliations:
1external, ou_persistent22              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: base editor, genome editing, prime editing, CRISPR/Cas, TALE, mitochondrion
 Abstract: Genome editing continues to revolutionize biological research. Due to its simplicity and flexibility, CRISPR/Cas-based editing has become the preferred technology in most systems. Cas nucleases tolerate fusion to large protein domains, thus allowing combination of their DNA recognition properties with new enzymatic activities. Fusion to nucleoside deaminase or reverse transcriptase domains has produced base editors and prime editors that, instead of generating double-strand breaks in the target sequence, induce site-specific alterations of single (or a few adjacent) nucleotides. The availability of protein-only genome editing reagents based on transcription activator-like effectors has enabled the extension of base editing to the genomes of chloroplasts and mitochondria. In this review, we summarize currently available base editing methods for nuclear and organellar genomes. We highlight recent advances with improving precision, specificity, and efficiency and discuss current limitations and future challenges. We also provide a brief overview of applications in agricultural biotechnology and gene therapy.

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Language(s): eng - English
 Dates: 2022-07-162022-07-16
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.tig.2022.06.015
 Degree: -

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Title: Trends in Genetics
  Other : Trends Genet.
Source Genre: Journal
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Publ. Info: Elsevier Current Trends
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0168-9525
CoNE: https://pure.mpg.de/cone/journals/resource/954925485719