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  Nitrate respiration occurs throughout the depth of mucoid and non-mucoid Pseudomonas aeruginosa submerged agar colony biofilms including the oxic zone

Schwermer, C. U., de Beer, D., & Stoodley, P. (2022). Nitrate respiration occurs throughout the depth of mucoid and non-mucoid Pseudomonas aeruginosa submerged agar colony biofilms including the oxic zone. SCIENTIFIC REPORTS, 12(1): 8557. doi:10.1038/s41598-022-11957-4.

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Schwermer, Carsten Ulrich1, Author
de Beer, Dirk2, Author           
Stoodley, Paul1, Author
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1external, ou_persistent22              
2Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481711              

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Free keywords: MICROSENSOR; DIFFUSION; BACTERIAScience & Technology - Other Topics;
 Abstract: Pseudomonas aeruginosa is an opportunistic pathogen and well characterized biofilm former. P. aeruginosa forms strong oxygen gradients inside biofilms due to rapid oxygen respiration in the top layers and the poor solubility of oxygen coupled with diffusion limited transport. Transcriptomic evidence from in vitro and ex vivo sampling suggests that denitrification is occurring in biofilms in ostensibly oxic environments. It is hypothesized that in the presence of nitrate there is stratification with aerobic respiration occurring in the outer oxic layer and denitrification in the lower anoxic zone. We used submerged agar colony biofilms grown from mucoid (FRD1) and non-mucoid (PAO1) strains to simultaneously measure depth microprofiles of oxygen and nitrous oxide in the same colony with microelectrodes. Oxygen respiration occurred at the top of the colony as expected but denitrification occurred throughout the entire depth, even in the oxic region. Local denitrification rates were highly variable suggesting heterogenous metabolic activity within the colony. We also assessed the short-term influence of tobramycin on aerobic respiration within a PAO1 colony. Although there was an immediate reduction in respiration it was never completely arrested over a 2 h period. On tobramycin removal the oxygen gradient steadily reestablished, demonstrating immediate recovery of metabolic activity.

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Language(s): eng - English
 Dates: 2022-05-20
 Publication Status: Published online
 Pages: 11
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 Table of Contents: -
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Title: SCIENTIFIC REPORTS
Source Genre: Journal
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Publ. Info: HEIDELBERGER PLATZ 3, BERLIN, 14197, GERMANY : NATURE PORTFOLIO
Pages: - Volume / Issue: 12 (1) Sequence Number: 8557 Start / End Page: - Identifier: ISSN: 2045-2322