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  A general design of caging-group-free photoactivatable fluorophores for live-cell nanoscopy

Lincoln, R., Bossi, M. L., Remmel, M., D´Este, E., Butkevich, A. N., & Hell, S. W. (2022). A general design of caging-group-free photoactivatable fluorophores for live-cell nanoscopy. Nature Chemistry, 14, 1013-1020. doi:10.1038/s41557-022-00995-0.

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 Creators:
Lincoln, Richard1, Author           
Bossi, Mariano L.1, Author           
Remmel, Michael1, Author           
D´Este, Elisa2, Author           
Butkevich, Alexey N.1, Author           
Hell, Stefan W.1, Author           
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: Fluorescence imaging; Fluorescence spectroscopy; Fluorescent dyes; Single-molecule fluorescence; Super-resolution microscopy
 Abstract: The controlled switching of fluorophores between non-fluorescent and fluorescent states is central to every superresolution fluorescence microscopy (nanoscopy) technique, and the exploration of radically new switching mechanisms remains critical to boosting the performance of established, as well as emerging superresolution methods. Photoactivatable dyes offer significant improvements to many of these techniques, but often rely on photolabile protecting groups that limit their applications. Here we describe a general method to transform 3,6-diaminoxanthones into caging-group free photoactivatable fluorophores. These photoactivatable xanthones (PaX) assemble rapidly and cleanly into highly fluorescent, photo- and chemically stable pyronine dyes upon irradiation with light. The strategy is extendable to carbon- and silicon-bridged xanthone analogs, yielding a new family of photoactivatable labels spanning much of the visible spectrum. Our results demonstrate the versatility and utility of PaX dyes in fixed and live-cell fluorescence microscopy, and both coordinate-targeted stimulated emission depletion (STED) and coordinate-stochastic single-molecule localization superresolution microscopy (SMLM).

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Language(s): eng - English
 Dates: 2021-10-012022-06-092022-07-212022-09
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: No review
 Degree: -

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Title: Nature Chemistry
  Abbreviation : Nat. Chem.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 14 Sequence Number: - Start / End Page: 1013 - 1020 Identifier: ISSN: 1755-4330
CoNE: https://pure.mpg.de/cone/journals/resource/1755-4330