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  Crystallographic studies of rhodopsins: structure and dynamics

Grünbein, M. L., Nass Kovacs, G., Kloos, M., Gorel, A., Doak, R. B., Shoeman, R. L., et al. (2022). Crystallographic studies of rhodopsins: structure and dynamics. In Methods in Molecular Biology (pp. 147-168). New York, NY: Humana. doi:10.1007/978-1-0716-2329-9_7.

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MethMolBiol_2501_2022_147.pdf (Any fulltext), 367KB
 
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Grünbein, Marie Luise1, Author           
Nass Kovacs, Gabriela1, Author           
Kloos, Marco1, Author           
Gorel, Alexander1, Author           
Doak, R. Bruce1, Author           
Shoeman, Robert L.1, Author           
Barends, Thomas R. M.1, Author           
Schlichting, Ilme1, Author           
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: Nanocrystallography; Photoexcitation; Pump probe experiment; Retinal; Rhodopsin; SFX; Serial crystallography; Serial femtosecond crystallography; Time-resolved crystallography; X-ray free-electron laser; XFEL.
 Abstract: Crystal structures have provided detailed insight in the architecture of rhodopsin photoreceptors. Of particular interest are the protein-chromophore interactions that govern the light-induced retinal isomerization and ultimately induce the large structural changes important for the various biological functions of the family. The reaction intermediates occurring along the rhodopsin photocycle have vastly differing lifetimes, from hundreds of femtoseconds to milliseconds. Detailed insight at high spatial and temporal resolution can be obtained by time-resolved crystallography using pump-probe approaches at X-ray free-electron lasers. Alternatively, cryotrapping approaches can be used. Both the approaches are described, including illumination and sample delivery. The importance of appropriate photoexcitation avoiding multiphoton absorption is stressed.

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Language(s): eng - English
 Dates: 2022-07-212022
 Publication Status: Issued
 Pages: 22
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 Table of Contents: -
 Rev. Type: Peer
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Title: Methods in Molecular Biology
Source Genre: Series
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Publ. Info: New York, NY : Humana
Pages: - Volume / Issue: 2501 Sequence Number: - Start / End Page: 147 - 168 Identifier: ISBN: 978-1-0716-2328-2
ISSN: 1064-3745