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  Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission

Lipstein, N., Chang, S., Lin, K.-H., Lopez-Murcia, F. J., Neher, E., Taschenberger, H., et al. (2021). Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission. Neuron, 109, 3980-4000.e7. doi:10.1016/j.neuron.2021.09.054.

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 Creators:
Lipstein, N.1, Author           
Chang, S.1, Author           
Lin, K.-H., Author
Lopez-Murcia, F. J.1, Author           
Neher, E., Author
Taschenberger, H.1, Author           
Brose, N.1, Author           
Affiliations:
1Molecular neurobiology, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173659              

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 Abstract: During ongoing presynaptic action potential (AP) firing, transmitter release is limited by the availability of release-ready synaptic vesicles (SVs). The rate of SV recruitment (SVR) to release sites is strongly upregu- lated at high AP frequencies to balance SV consumption. We show that Munc13-1—an essential SV priming protein—regulates SVR via a Ca2+-phospholipid-dependent mechanism. Using knockin mouse lines with point mutations in the Ca2+-phospholipid-binding C2B domain of Munc13-1, we demonstrate that abolishing Ca2+-phospholipid binding increases synaptic depression, slows recovery of synaptic strength after SV pool depletion, and reduces temporal fidelity of synaptic transmission, while increased Ca2+-phospholipid binding has the opposite effects. Thus, Ca2+-phospholipid binding to the Munc13-1-C2B domain accelerates SVR, reduces short-term synaptic depression, and increases the endurance and temporal fidelity of neurotrans- mission, demonstrating that Munc13-1 is a core vesicle priming hub that adjusts SV re-supply to demand.

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Language(s): eng - English
 Dates: 2021-12-15
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.neuron.2021.09.054
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Project name : This work was funded by the European Commission (ERC-AdG SynPrime, N.B.) and the German Research Foundation (CNMPB, H.T. and N.B.; EXC2067/1-390729940, N.B.; SFB1286, E.N., N.B., and N.L.).
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Project name : SYNPRIME
Grant ID : 670283
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: Neuron
Source Genre: Journal
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Pages: - Volume / Issue: 109 Sequence Number: - Start / End Page: 3980 - 4000.e7 Identifier: ISSN: 08966273