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  Serial femtosecond crystallography

Barends, T. R. M., Stauch, B., Cherezov, V., & Schlichting, I. (2022). Serial femtosecond crystallography. Nature Reviews Methods primers, 2: 59, pp. 1-24. doi:10.1038/s43586-022-00141-7.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-D098-F Version Permalink: https://hdl.handle.net/21.11116/0000-000A-D099-E
Genre: Journal Article

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 Creators:
Barends, Thomas R. M.1, Author           
Stauch, Benjamin, Author
Cherezov, Vadim, Author
Schlichting, Ilme1, Author           
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: With the advent of X-ray free-electron lasers (XFELs), new, high-throughput serial crystallography techniques for macromolecular structure determination have emerged. Serial femtosecond crystallography (SFX) and related methods provide possibilities beyond canonical, single-crystal rotation crystallography by mitigating radiation damage and allowing time-resolved studies with unprecedented temporal resolution. This Primer aims to assist structural biology groups with little or no experience in serial crystallography in planning and carrying out a successful SFX experiment. It discusses the background of serial crystallography and its possibilities. Microcrystal growth and characterization methods are discussed, alongside techniques for sample delivery and data processing. Moreover, it gives practical tips for preparing an experiment, what to consider and do during a beam time and how to conduct the final data analysis. Finally, the Primer looks at various applications of SFX, including structure determination of membrane proteins, investigation of radiation damage-prone systems and time-resolved studies.

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Language(s): eng - English
 Dates: 2022-06-142022-08-04
 Publication Status: Published online
 Pages: 24
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s43586-022-00141-7
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Title: Nature Reviews Methods primers
  Abbreviation : Nat. Rev. Methods Primers
Source Genre: Journal
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Publ. Info: London : Nature Research
Pages: - Volume / Issue: 2 Sequence Number: 59 Start / End Page: 1 - 24 Identifier: ISSN: 2662-8449
CoNE: https://pure.mpg.de/cone/journals/resource/2662-8449