日本語
 
Help Privacy Policy ポリシー/免責事項
  詳細検索ブラウズ

アイテム詳細

  Canalization of genome-wide transcriptional activity in Arabidopsis thaliana accessions by MET1-dependent CG methylation

Srikant, T., Yuan, W., Berendzen, K., Contreras Garrido, A., Drost, H.-G., Schwab, R., & Weigel, D. (2022). Canalization of genome-wide transcriptional activity in Arabidopsis thaliana accessions by MET1-dependent CG methylation. Genome Biology, 23(1):. doi:10.1186/s13059-022-02833-5.

Item is

基本情報

表示: 非表示:
アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-000A-D5D3-7 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000C-E11E-5
資料種別: 学術論文

ファイル

表示: ファイル

関連URL

表示:

作成者

表示:
非表示:
 作成者:
Srikant, T1, 著者           
Yuan, W1, 著者           
Berendzen, KW, 著者
Contreras Garrido, A1, 著者           
Drost, H-G1, 2, 著者           
Schwab, R1, 3, 著者                 
Weigel, D1, 著者           
所属:
1Department Molecular Biology, Max Planck Institute for Biology Tübingen, Max Planck Society, ou_3371687              
2Computational Biology Group, Department Molecular Biology, Max Planck Institute for Biology Tübingen, Max Planck Society, ou_3496867              
3Research Group Ecological Genetics, Department Molecular Biology, Max Planck Institute for Biology Tübingen, Max Planck Society, ou_3502746              

内容説明

表示:
非表示:
キーワード: -
 要旨: Background: Despite its conserved role on gene expression and transposable element (TE) silencing, genome-wide CG methylation differs substantially between wild Arabidopsis thaliana accessions.
Results: To test our hypothesis that global reduction of CG methylation would reduce epigenomic, transcriptomic, and phenotypic diversity in A. thaliana accessions, we knock out MET1, which is required for CG methylation, in 18 early-flowering accessions. Homozygous met1 mutants in all accessions suffer from common developmental defects such as dwarfism and delayed flowering, in addition to accession-specific abnormalities in rosette leaf architecture, silique morphology, and fertility. Integrated analysis of genome-wide methylation, chromatin accessibility, and transcriptomes confirms that MET1 inactivation greatly reduces CG methylation and alters chromatin accessibility at thousands of loci. While the effects on TE activation are similarly drastic in all accessions, the quantitative effects on non-TE genes vary greatly. The global expression profiles of accessions become considerably more divergent from each other after genome-wide removal of CG methylation, although a few genes with diverse expression profiles across wild-type accessions tend to become more similar in mutants. Most differentially expressed genes do not exhibit altered chromatin accessibility or CG methylation in cis, suggesting that absence of MET1 can have profound indirect effects on gene expression and that these effects vary substantially between accessions.
Conclusions: Systematic analysis of MET1 requirement in different A. thaliana accessions reveals a dual role for CG methylation: for many genes, CG methylation appears to canalize expression levels, with methylation masking regulatory divergence. However, for a smaller subset of genes, CG methylation increases expression diversity beyond genetically encoded differences.

資料詳細

表示:
非表示:
言語:
 日付: 2022-12
 出版の状態: オンラインで出版済み
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): DOI: 10.1186/s13059-022-02833-5
PMID: 36539836
 学位: -

関連イベント

表示:

訴訟

表示:

Project information

表示:

出版物 1

表示:
非表示:
出版物名: Genome Biology
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: London : BioMed Central Ltd.
ページ: 33 巻号: 23 (1) 通巻号: 263 開始・終了ページ: - 識別子(ISBN, ISSN, DOIなど): ISSN: 1465-6906
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000224390_1