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  Crystal structure of a dimeric archaeal cleavage and polyadenylation specificity factor

Mir-Montazeri, B., Ammelburg, M., Forouzan, D., Lupas, A., & Hartmann, M. (2011). Crystal structure of a dimeric archaeal cleavage and polyadenylation specificity factor. Journal of Structural Biology, 173(1), 191-195. doi:10.1016/j.jsb.2010.09.013.

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Mir-Montazeri, B1, Author           
Ammelburg, M1, Author           
Forouzan, D1, Author           
Lupas, AN1, Author           
Hartmann, MD1, Author           
Affiliations:
1Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375791              

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 Abstract: Proteins of the metallo-β-lactamase (MβL) fold form a large superfamily of metallo-hydrolase/oxidoreductases. Members of this family are found in all three domains of life and are involved in a variety of biological functions related to hydrolysis, redox processes, DNA repair and uptake, and RNA processing. We classified the archaeal homologs of this superfamily based on sequence similarity and characterized a subfamily of the Cleavage and Polyadenylation Specificity Factor (CPSF) with an uncommon domain composition: in addition to an extended MβL domain, which accommodates the active site for RNA cleavage, this group has two N-terminal KH domains. Here, we present the crystal structure of a member of this group from Methanosarcina mazei. It reveals a dimerization mode of the MβL domain that has not been observed before and suggests that RNA is bound across the dimer interface, recognized by the KH domains of one monomer, and cleaved at the active site of the other.

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 Dates: 2011-01
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.jsb.2010.09.013
PMID: 20851187
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Title: Journal of Structural Biology
  Abbreviation : J. Struct. Biol.
Source Genre: Journal
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Publ. Info: San Diego, CA : Elsevier
Pages: - Volume / Issue: 173 (1) Sequence Number: - Start / End Page: 191 - 195 Identifier: ISSN: 1047-8477
CoNE: https://pure.mpg.de/cone/journals/resource/954922650160