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  Determination of atomoxetine or escitalopram in human plasma by HPLC: Applications in neuroscience research studies

Teichert, J., Rowe, J. B., Ersche, K. D., Skandali, N., Sacher, J., Aigner, A., et al. (2020). Determination of atomoxetine or escitalopram in human plasma by HPLC: Applications in neuroscience research studies. International Journal of Clinical Pharmacology and Therapeutics, 58(8), 426-438. doi:10.5414/CP203705.

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 Creators:
Teichert, Jens1, Author
Rowe, James B.2, 3, Author
Ersche, Karen D.4, Author
Skandali, Nikolina2, 4, 5, Author
Sacher, Julia6, 7, 8, Author           
Aigner, Achim1, Author
Regenthal, Ralf1, Author
Affiliations:
1Division of Clinical Pharmacology, Rudolf-Boehm-Institute of Pharmacology and Toxicology, University of Leipzig, Germany, ou_persistent22              
2Behavioural and Clinical Neuroscience Institute, University of Cambridge, United Kingdom, ou_persistent22              
3Department of Clinical Neuroscience, University of Cambridge, United Kingdom, ou_persistent22              
4Department of Psychiatry, University of Cambridge, United Kingdom, ou_persistent22              
5Cambridgeshire and Peterborough NHS Foundation Trust, Cambridge, United Kingdom, ou_persistent22              
6Minerva Research Group EGG (Emotion & neuroimaGinG) Lab, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_3230775              
7Department Neurology, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634549              
8Clinic for Cognitive Neurology, University of Leipzig, Germany, ou_persistent22              

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Free keywords: Atomoxetine; Escitalopram; HPLC Method Neuroscience Application
 Abstract: Background: Atomoxetine and escitalopram are potent and selective drugs approved for noradrenergic or serotonergic modulation of neuronal networks in attentiondeficit hyperactivity disorder (ADHD) or depression, respectively. High-performance liquid chromatography (HPLC) methods still play an important role in the therapeutic drug monitoring (TDM) of psychopharmacological drugs, and coupled with tandem mass spectrometry are the gold standard for the quantification of drugs in biological matrices, but not available everywhere. The aim of this work was to develop and validate a HPLC method for neuroscientific studies using atomoxetine or escitalopram as a test drug. Materials and methods: A HPLC method from routine TDM determination of atomoxetine or citalopram in plasma was adapted and validated for use in neuroscientific research. Using photo diode array detection with UV absorption at 205 nm, the variation of internal standard within one chromatographic method enables separate drug monitoring for concentration-controlled explorative studies in healthy humans and patients with Parkinson's disease. Results: The method described here was found to be linear in the range of 0.002 - 1.4 mg/L for atomoxetine and 0.0012 - 0.197 mg/L for escitalopram, with overall mean intra-day and interday imprecision and accuracy bias < 10% for both drugs. The method was successfully applied in concentration-controlled neuroimaging studies in populations of healthy humans and patients with Parkinson's disease. Conclusion: A simple, sensitive, robust HPLC method capable of monitoring escitalopram and atomoxetine is presented and validated, as a useful tool for drug monitoring and the study of pharmacokinetics in neuroscientific study applications.

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Language(s): eng - English
 Dates: 2020-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.5414/CP203705
 Degree: -

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Title: International Journal of Clinical Pharmacology and Therapeutics
Source Genre: Journal
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Publ. Info: München-Deisenhofen : Dustri-Verlag Dr. Karl Feistle
Pages: - Volume / Issue: 58 (8) Sequence Number: - Start / End Page: 426 - 438 Identifier: ISSN: 0946-1965
CoNE: https://pure.mpg.de/cone/journals/resource/954925572918