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  Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers

Holmes, S., Kirkwood, H. J., Bean, R., Giewekemeyer, K., Martin, A. V., Hadian-Jazi, M., et al. (2022). Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers. Nature Communications, 13(1): 4708. doi:10.1038/s41467-022-32434-6.

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 Creators:
Holmes, S.1, Author
Kirkwood, H. J.1, Author
Bean, R.1, Author
Giewekemeyer, K.1, Author
Martin, A. V.1, Author
Hadian-Jazi, M.1, Author
Wiedorn, M. O.1, Author
Oberthür, D.1, Author
Marman, H.1, Author
Adriano, L.1, Author
Al-Qudami, N.1, Author
Bajt, S.1, Author
Barák, I.1, Author
Bari, S.1, Author
Bielecki, J.1, Author
Brockhauser, S.1, Author
Coleman, M. A.1, Author
Cruz-Mazo, F.1, Author
Danilevski, C.1, Author
Dörner, K.1, Author
Gañán-Calvo, A. M.1, AuthorGraceffa, R.1, AuthorFangohr, H.2, 3, 4, AuthorHeymann, M.1, AuthorFrank, M.1, AuthorKaukher, A.1, AuthorKim, Y.1, AuthorKobe, B.1, AuthorKnoška, J.1, AuthorLaurus, T.1, AuthorLetrun, R.1, AuthorMaia, L.1, AuthorMesserschmidt, M.1, AuthorMetz, M.1, AuthorMichelat, T.1, AuthorMills, G.1, AuthorMolodtsov, S.1, AuthorMonteiro, D. C. F.1, AuthorMorgan, A. J.1, AuthorMünnich, A.1, AuthorMurillo, G. E. P.1, AuthorPrevitali, G.1, AuthorRound, A.1, AuthorSato, T.1, AuthorSchubert, R.1, AuthorSchulz, J.1, AuthorShelby, M.1, AuthorSeuring, C.1, AuthorSellberg, J. A.1, AuthorSikorski, M.1, AuthorSilenzi, A.1, AuthorStern, S.1, AuthorSztuk-Dambietz, J.1, AuthorSzuba, J.1, AuthorTrebbin, M.1, AuthorVagovic, P.1, AuthorVe, T.1, AuthorWeinhausen, B.1, AuthorWrona, K.1, AuthorPaulraj, L. X.5, Author           Xu, C.1, AuthorYefanov, O.1, AuthorNugent, K. A.1, AuthorChapman, H. N.1, AuthorMancuso, A. P.1, AuthorBarty, A.1, AuthorAbbey, B.1, AuthorDarmanin, C.1, Author more..
Affiliations:
1external, ou_persistent22              
2European XFEL, ou_persistent22              
3Computational Science, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_3267028              
4University of Southampton, ou_persistent22              
5International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              

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 Abstract: The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.

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Language(s): eng - English
 Dates: 2019-05-232022-07-282022-08-11
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-022-32434-6
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 13 (1) Sequence Number: 4708 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723