ausblenden:
Schlagwörter:
Bacterial Proteins/*metabolism
Blotting, Western
Chemotaxis/*physiology
Cryoelectron Microscopy
Escherichia coli/metabolism/*physiology
Escherichia coli Proteins
Fluorescence Resonance Energy Transfer
Histidine Kinase
Ligands
Membrane Proteins/*metabolism
Methyl-Accepting Chemotaxis Proteins
*Models, Molecular
Multiprotein Complexes/*metabolism/physiology
Signal Transduction/*physiology
Zusammenfassung:
In chemotactic bacteria, transmembrane chemoreceptors, CheA and CheW form the core signalling complex of the chemotaxis sensory apparatus. These complexes are organized in extended arrays in the cytoplasmic membrane that allow bacteria to respond to changes in concentration of extracellular ligands via a cooperative, allosteric response that leads to substantial amplification of the signal induced by ligand binding. Here, we have combined cryo-electron tomographic studies of the 3D spatial architecture of chemoreceptor arrays in intact E. coli cells with computational modelling to develop a predictive model for the cooperativity and sensitivity of the chemotaxis response. The predictions were tested experimentally using fluorescence resonance energy transfer (FRET) microscopy. Our results demonstrate that changes in lateral packing densities of the partially ordered, spatially extended chemoreceptor arrays can modulate the bacterial chemotaxis response, and that information about the molecular organization of the arrays derived by cryo-electron tomography of intact cells can be translated into testable, predictive computational models of the chemotaxis response.
