English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Advances in crossover localization and QTL mapping using high-throughput sequencing

Rowan, B., Patel, V., Weigel, D., & Schneeberger, K. (2014). Advances in crossover localization and QTL mapping using high-throughput sequencing. In 25th International Conference on Arabidopsis Research (ICAR 2014) (pp. 176).

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-000A-E011-5 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-E012-4
Genre: Meeting Abstract

Files

show Files

Locators

show
hide
Locator:
http://www.arabidopsisresearch.org/images/ICAR/25th_ICAR_2014Abstracts.pdf (Abstract)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Rowan, B1, Author           
Patel, V, Author
Weigel, D1, Author           
Schneeberger, K, Author           
Affiliations:
1Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375790              

Content

show
hide
Free keywords: -
 Abstract: Crossovers (COs) that form between homologous chromosomes during meiosis allow for novel combinations of
alleles in progeny and can therefore generate trait variation that did not exist in the parental generation. The
frequency and spatial location of COs are not only important for generating the raw material for evolution, but also
contribute to our ability to perform quantitative trait locus (QTL) mapping for phenotypic traits. Understanding the
mechanisms that govern CO formation will inform our current views of its importance for evolution and also
provide potential targets for facilitating plant breeding. Engineering changes in the frequency and distribution of
COs can improve QTL mapping of important agricultural traits and an also allow for more efficient trait
introgression by reducing the number of linked loci. Here we describe improvements in the ability to precisely
locate crossover events and perform QTL mapping using low-coverage whole-genome sequencing of individuals
in mapping populations. We developed a method for preparing libraries for next-generation sequencing at about a
tenth of the cost of established protocols and then used a refined Hidden Markov Model approach to reconstruct
the recombinant genomes of hundreds of F2 individuals generated from the Col-0 and Ws-2 accessions of
Arabidopsis thaliana. We resolved most CO breakpoints to within 2 kb and detected a 25-kb QTL interval for
flowering time on Chromosome 5 that includes the MAF2-5 gene cluster, which has previously been shown to
contribute to flowering time variation in natural populations. We conclude that this approach is efficient for
studying genetic factors affecting the crossover landscape and allows for the simultaneous (fine-) mapping of
quantitative traits.

Details

show
hide
Language(s):
 Dates: 2014-07
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: -
 Degree: -

Event

show
hide
Title: 25th International Conference on Arabidopsis Research (ICAR 2014)
Place of Event: Vancouver, Canda
Start-/End Date: 2014-07-28 - 2014-08-01

Legal Case

show

Project information

show

Source 1

show
hide
Title: 25th International Conference on Arabidopsis Research (ICAR 2014)
Source Genre: Proceedings
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: 375 Start / End Page: 176 Identifier: -