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  Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor

Karki, P., Carney, T. D., Maracci, C., Yatsenko, A. S., Shcherbata, H. R., & Rodnina, M. V. (2022). Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor. Nucleic Acids Research, 50(11), 6001-6019. doi:10.1093/nar/gkab1189.

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 Creators:
Karki, P.1, Author           
Carney, T. D., Author
Maracci, C.1, Author           
Yatsenko, A. S., Author
Shcherbata, H. R., Author
Rodnina, M. V.1, Author           
Affiliations:
1Department of Physical Biochemistry, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350156              

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Free keywords: CRISPR/Cas9 , Drosophila melanogaster, eRF1H
 Abstract: Translational readthrough (TR) occurs when the ribosome decodes a stop codon as a sense codon,
resulting in two protein isoforms synthesized from
the same mRNA. TR has been identified in several
eukaryotic organisms; however, its biological significance and mechanism remain unclear. Here, we
quantify TR of several candidate genes in
Drosophila
melanogaster and characterize the regulation of TR
in the large Maf transcription factor Traffic jam
(Tj). Using CRISPR/Cas9-generated mutant flies, we
show that the TR-generated Tj isoform is expressed
in a subset of neural cells of the central nervous
system and is excluded from the somatic cells of
gonads. Control of TR in Tj is critical for preservation of neuronal integrity and maintenance of reproductive health. The tissue-specific distribution of a
release factor splice variant, eRF1H, plays a critical
role in modulating differential TR of leaky stop codon
contexts. Finetuning of gene regulatory functions
of transcription factors by TR provides a potential
mechanism for cell-specific regulation of gene ex-pression.

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Language(s): eng - English
 Dates: 2021-11-052021-12-062021-12-132022
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1093/nar/gkab1189
 Degree: -

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Project name : The work was supported by a grant of the Deutsche Forschungsgemeinschaft (SFB860 and Leibniz Prize to M.V.R.) and VolkswagenStiftung (Az. 97750 to H.R.S.).
Grant ID : -
Funding program : -
Funding organization : DFG

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Title: Nucleic Acids Research
Source Genre: Journal
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Publ. Info: Nucleic Acids Research
Pages: - Volume / Issue: 50 (11) Sequence Number: - Start / End Page: 6001 - 6019 Identifier: PMID: 34897510