日本語
 
Help Privacy Policy ポリシー/免責事項
  詳細検索ブラウズ

アイテム詳細

登録内容を編集ファイル形式で保存
EndNote (UTF-8)
 
ダウンロード電子メール
  A transition from strong right-handed to canonical left-handed supercoiling in a conserved coiled-coil segment of trimeric autotransporter adhesins

Hernandez Alvarez, B., Gruber, M., Ursinus, A., Dunin-Horkawicz, S., Lupas, A., & Zeth, K. (2010). A transition from strong right-handed to canonical left-handed supercoiling in a conserved coiled-coil segment of trimeric autotransporter adhesins. Journal of Structural Biology, 170(2), 236-245. doi:10.1016/j.jsb.2010.02.009.

Item is

基本情報

非表示:
アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-000A-E2A0-1 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000B-A532-2
資料種別: 学術論文

ファイル

表示: ファイル

関連URL

表示:

作成者

非表示:
 作成者:
Hernandez Alvarez, B1, 2, 著者           
Gruber, M1, 著者           
Ursinus, A1, 著者           
Dunin-Horkawicz, S1, 著者           
Lupas, AN1, 著者           
Zeth, K1, 著者           
所属:
1Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375791              
2Conservation of Protein Structure and Function Group, Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3477389              

内容説明

非表示:
キーワード: -
 要旨: Trimeric autotransporter adhesins (TAAs) represent an important class of pathogenicity factors in proteobacteria. Their defining feature is a conserved membrane anchor, which forms a 12-stranded beta-barrel through the outer membrane. The proteins are translocated through the pore of this barrel and, once export is complete, the pore is occluded by a three-stranded coiled coil with canonical heptad (7/2) sequence periodicity. In many TAAs this coiled coil is extended by a segment of varying length, which has pentadecad (15/4) periodicity. We used X-ray crystallography and biochemical methods to analyze the transition between these two periodicities in the coiled-coil stalk of the Yersinia adhesin YadA. Our results show how the strong right-handed supercoil of the 15/4-periodic part locally undergoes further over-winding to 19/5, before switching at a fairly constant rate over 14 residues to the canonical left-handed supercoil of the 7/2-periodic part. The transition region contains two YxD motifs, which are characteristic for right-handed coiled-coil segments of TAAs. This novel coiled-coil motif forms a defined network of inter- and intrahelical hydrogen bonds, thus serving as a structural determinant. Supercoil fluctuations have hitherto been described in coiled coils whose main sequence periodicity is disrupted locally by discontinuities. Here we present the first detailed analysis of two fundamentally different coiled-coil periodicities being accommodated in the same structure.

資料詳細

非表示:
言語:
 日付: 2010-05
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): DOI: 10.1016/j.jsb.2010.02.009
PMC: 20178846
 学位: -

関連イベント

表示:

訴訟

表示:

Project information

表示:

出版物 1

非表示:
出版物名: Journal of Structural Biology
  省略形 : J. Struct. Biol.
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: San Diego, CA : Elsevier
ページ: - 巻号: 170 (2) 通巻号: - 開始・終了ページ: 236 - 245 識別子(ISBN, ISSN, DOIなど): ISSN: 1047-8477
CoNE: https://pure.mpg.de/cone/journals/resource/954922650160