English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Detergents: an overview

Linke, D. (2009). Detergents: an overview. In Methods in Enzymology. New York, NY: Academic Press.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Linke, D1, Author           
Affiliations:
1Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375791              

Content

show
hide
Free keywords: -
 Abstract: Detergents are used in molecular biology laboratories every day. They are present in cell lysis buffers (e.g., in kits for plasmid isolation), in electrophoresis and blotting buffers, and, most importantly, they are used for cleaning laboratory glassware and the hands of the laboratory staff. For these routine applications, a detailed knowledge of detergent properties is not necessary-they just work. When it comes to the isolation and purification of membrane proteins, one cannot rely on routine protocols. Many membrane proteins are only stable in a small number of different detergent buffer systems, and worst of all, different membrane proteins prefer very different detergents. Unfortunately, detergent properties are considered the domain of colloid science or physical chemistry, and thus, while the available amount of physico-chemical data on detergents is astounding, this data is rarely compiled in a way that is useful to biochemists. The aim of this chapter is to provide an overview of the physical and chemical properties of detergents commonly used in membrane protein science and to explain how these properties can be exploited for protein purification.

Details

show
hide
Language(s):
 Dates: 2009
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/S0076-6879(09)63034-2
PMID: 19892194
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Methods in Enzymology
  Other : Methods Enzymol.
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Academic Press
Pages: - Volume / Issue: 463 Sequence Number: - Start / End Page: - Identifier: ISSN: 0076-6879
CoNE: https://pure.mpg.de/cone/journals/resource/110975506069301

Source 2

show
hide
Title: Guide to Protein Purification
Source Genre: Book
 Creator(s):
Burgess, RR, Editor
Deutscher, MP, Editor
Affiliations:
-
Publ. Info: San Diego, CA, USA : Academic Press, 2nd Edition
Pages: 851 Volume / Issue: - Sequence Number: - Start / End Page: 603 - 617 Identifier: ISBN: 978-0-12-374536-1