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  In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains

Van den Hoek, H., Klena, N., Jordan, M. A., Viar, G. A., Righetto, R. D., Schaffer, M., et al. (2022). In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains. Science, 377(6605), 543-548. doi:10.1126/science.abm6704.

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 Creators:
Van den Hoek, Hugo1, Author           
Klena, Nikolai2, Author
Jordan, Mareike A.2, Author
Viar, Gonzalo Alvarez2, Author
Righetto, Ricardo D.2, Author
Schaffer, Miroslava1, Author           
Erdmann, Philipp S.2, Author
Wan, William2, Author
Geimer, Stefan2, Author
Plitzko, Jürgen M.1, Author           
Baumeister, Wolfgang1, Author           
Pigino, Gaia2, Author
Hamel, Virginie2, Author
Guichard, Paul2, Author
Engel, Benjamin D.2, Author
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2external, ou_persistent22              

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Free keywords: MEMBRANE; PROTEIN; IFT; ASYMMETRY; PARTICLES; BARRIER; DYNEINScience & Technology - Other Topics;
 Abstract: The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains a selective barrier that regulates the entry of large intraflagellar transport (IFT) trains, which carry cargo proteins required for ciliary assembly and maintenance. However, the native architecture of the ciliary base and the process of IFT train assembly remain unresolved. In this work, we used in situ cryo-electron tomography to reveal native structures of the transition zone region and assembling IFT trains at the ciliary base in Chlamydomonas. We combined this direct cellular visualization with ultrastructure expansion microscopy to describe the front-to-back stepwise assembly of IFT trains: IFT-B forms the backbone, onto which bind IFT-A, dynein-1b, and finally kinesin-2 before entry into the cilium.

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Language(s): eng - English
 Dates: 2022-06-27
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000836647500044
DOI: 10.1126/science.abm6704
 Degree: -

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Title: Science
  Abbreviation : Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 377 (6605) Sequence Number: - Start / End Page: 543 - 548 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1