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  Strategies for Proteome-Wide Quantification of Glycosylation Macro- and Micro-Heterogeneity

Fang, P., Ji, Y., Oellerich, T., Urlaub, H., & Pan, K.-T. (2022). Strategies for Proteome-Wide Quantification of Glycosylation Macro- and Micro-Heterogeneity. International Journal of Molecular Sciences, 23(3): 1609. doi:10.3390/ijms23031609.

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ijms-23-01609-v3.pdf (Publisher version), 2MB
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 Creators:
Fang, Pan, Author
Ji, Yanlong1, Author           
Oellerich, Thomas, Author
Urlaub, Henning1, Author           
Pan, Kuan-Ting, Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350290              

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Free keywords: glycoproteomics; quantification; mass spectrometry; stable-isotope labeling; label free
 Abstract: Protein glycosylation governs key physiological and pathological processes in human cells. Aberrant glycosylation is thus closely associated with disease progression. Mass spectrometry (MS)-based glycoproteomics has emerged as an indispensable tool for investigating glycosylation changes in biological samples with high sensitivity. Following rapid improvements in methodologies for reliable intact glycopeptide identification, site-specific quantification of glycopeptide macro- and micro-heterogeneity at the proteome scale has become an urgent need for exploring glycosylation regulations. Here, we summarize recent advances in N- and O-linked glycoproteomic quantification strategies and discuss their limitations. We further describe a strategy to propagate MS data for multilayered glycopeptide quantification, enabling a more comprehensive examination of global and site-specific glycosylation changes. Altogether, we show how quantitative glycoproteomics methods explore glycosylation regulation in human diseases and promote the discovery of biomarkers and therapeutic targets.

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Language(s): eng - English
 Dates: 2022-01-30
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/ijms23031609
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Title: International Journal of Molecular Sciences
  Abbreviation : Int. J. Mol. Sci.
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 23 (3) Sequence Number: 1609 Start / End Page: - Identifier: ISSN: 1422-0067
CoNE: https://pure.mpg.de/cone/journals/resource/1422-0067