English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  DNA-PAINT MINFLUX nanoscopy

Ostersehlt, L.-M., Jans, D. C., Wittek, A., Keller-Findeisen, J., Inamdar, K., Sahl, S., et al. (2022). DNA-PAINT MINFLUX nanoscopy. Nature Methods, 19, 1072-1075. doi:10.1038/s41592-022-01577-1.

Item is

Files

show Files
hide Files
:
3406193.pdf (Publisher version), 5MB
Name:
3406193.pdf
Description:
-
OA-Status:
Gold
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Ostersehlt, Lynn-Marie1, Author                 
Jans, Daniel C.2, Author                 
Wittek, Anna2, Author                 
Keller-Findeisen, Jan1, Author           
Inamdar, Kaushik1, Author                 
Sahl, Steffen1, Author           
Hell, Stefan W.1, Author                 
Jakobs, Stefan1, Author                 
Affiliations:
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350048              
2Research Group of Mitochondrial Structure and Dynamics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350049              

Content

show
hide
Free keywords: Nanoscale biophysics Super-resolution microscopy
 Abstract: MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations,
has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching
process for single fluorophore separation, initial MINFLUX
implementations have been limited to two color channels.
Here we show that MINFLUX can be effectively combined with
sequentially multiplexed DNA-based labeling (DNA-PAINT),
expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings
of mitochondria in human cells.

Details

show
hide
Language(s): eng - English
 Dates: 2022-09-01
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-022-01577-1
 Degree: -

Event

show

Legal Case

show

Project information

show hide
Project name : This work was supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2067/1-390729940 (to S.W.H. and S.J.) and by the European Research Council (ERC AdG no. 835102) (to S.J.). The MINFLUX system was funded by the DFG (grant no. INST 186/1303-1 to S.J.). The research was funded by the DFG-funded TRR 274 (project no. Z01) and SFB 1190 (project no. P01).
Grant ID : -
Funding program : -
Funding organization : -

Source 1

show
hide
Title: Nature Methods
  Other : Nature Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Nature Publishing Group
Pages: - Volume / Issue: 19 Sequence Number: - Start / End Page: 1072 - 1075 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556