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Nanoscale biophysics
Super-resolution microscopy
Abstract:
MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations,
has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching
process for single fluorophore separation, initial MINFLUX
implementations have been limited to two color channels.
Here we show that MINFLUX can be effectively combined with
sequentially multiplexed DNA-based labeling (DNA-PAINT),
expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings
of mitochondria in human cells.