Quick miniprep for plant DNA isolation

Weigel, D; Glazebrook, J

doi:10.1101/pdb.prot5179

Local TagsRelease HistoryDetailsSummary

Quick miniprep for plant DNA isolation

Weigel, D., & Glazebrook, J. (2009). Quick miniprep for plant DNA isolation. Cold Spring Harbor protocols, 4(3): pdb.prot5179. doi:10.1101/pdb.prot5179.

Item is Released

show all hide all

Basic

show hide

Item Permalink: https://hdl.handle.net/21.11116/0000-000B-0EED-C Version Permalink: https://hdl.handle.net/21.11116/0000-000B-128D-2

Genre: Journal Article

Files

show Files

Locators

show

Creators

show

hide

Creators:
Weigel, D¹, Author
Glazebrook, J, Author

Affiliations:
1Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375790

Content

show

hide

Free keywords: -

Abstract: This procedure describes a quick miniprep for plant DNA isolation. It yields a small quantity of poorly purified DNA. However, many samples can be processed in a short time. When used with Arabidopsis, the method generally provides DNA of sufficient quality for polymerase chain reaction (PCR) amplification of cleaved amplified polymorphic sequences (CAPS) and simple sequence length polymorphism (SSLP) markers. Repeated freeze-thaw cycles should be avoided, because they result in samples that will no longer work as PCR templates.

Details

show

hide

Language(s):

Dates: Date issued: 2009-03

Publication Status: Issued

Pages: -

Publishing info: -

Table of Contents: -

Rev. Type: -

Identifiers: DOI: 10.1101/pdb.prot5179
PMID: 20147114

Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show

hide

Title: Cold Spring Harbor protocols

Abbreviation : CSH Protocols

Source Genre: Journal

Creator(s):

Affiliations:

Publ. Info: NY : Cold Spring Harbor

Pages: 3 Volume / Issue: 4 (3) Sequence Number: pdb.prot5179 Start / End Page: - Identifier: ISSN: 1559-6095
CoNE: https://pure.mpg.de/cone/journals/resource/1559-6095