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  Genetic analysis of Arabidopsis mutants

Weigel, D., & Glazebrook, J. (2008). Genetic analysis of Arabidopsis mutants. Cold Spring Harbor protocols, 3(3): pdb.top35. doi:10.1101/pdb.top35.

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Weigel, D1, Author                 
Glazebrook, J, Author
Affiliations:
1Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375790              

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 Abstract: The inheritance of mutant phenotypes in Arabidopsis thaliana is most often analyzed in the progeny resulting from crosses between different parents. This article describes genetic strategies for the analysis of mutants. Newly identified mutations can be examined initially using segregation analysis, followed by backcrossing and cosegregation for removal of extraneous mutations and assessment of pleiotropy. Confirmation that a phenotype of interest results from a given mutation can be achieved via complementation testing, which is also used to determine allelism of recessive mutations. The construction of double mutants is useful in the study of signal transduction and metabolic pathways. Considerations for the use of double homozygotes and their identification in the F(2) and F(3) generations are described at the end of this article.

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 Dates: 2008-03
 Publication Status: Issued
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.1101/pdb.top35
PMID: 21356798
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Title: Cold Spring Harbor protocols
  Abbreviation : CSH Protocols
Source Genre: Journal
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Publ. Info: NY : Cold Spring Harbor
Pages: 7 Volume / Issue: 3 (3) Sequence Number: pdb.top35 Start / End Page: - Identifier: ISSN: 1559-6095
CoNE: https://pure.mpg.de/cone/journals/resource/1559-6095