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  The TOR complex controls ATP levels to regulate actin cytoskeleton dynamics in Arabidopsis

Dai, L., Wang, B., Wang, T., Meyer, E. H., Kettel, V., Hoffmann, N., et al. (2022). The TOR complex controls ATP levels to regulate actin cytoskeleton dynamics in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 119(38): e2122969119. doi:10.1073/pnas.2122969119.

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Genre: Journal Article
Alternative Title : Proceedings of the National Academy of Sciences

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 Creators:
Dai, Liufeng1, Author
Wang, Baojie1, Author
Wang, Ting1, Author
Meyer, Etienne H.1, Author
Kettel, Valentin1, Author
Hoffmann, Natalie1, Author
McFarlane, Heather E.1, Author
Li, Shalan1, Author
Wu, Xuna1, Author
Picard, Kelsey L.1, Author
Giavalisco, Patrick1, Author
Persson, Staffan1, Author
Zhang, Y.2, Author           
Affiliations:
1external, ou_persistent22              
2Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              

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 Abstract: Energy is essential for all cellular functions in a living organism. How cells coordinate their physiological processes with energy status and availability is thus an important question. The turnover of actin cytoskeleton between its monomeric and filamentous forms is a major energy drain in eukaryotic cells. However, how actin dynamics are regulated by ATP levels remain largely unknown in plant cells. Here, we observed that seedlings with impaired functions of target of rapamycin complex 1 (TORC1), either by mutation of the key component, RAPTOR1B, or inhibition of TOR activity by specific inhibitors, displayed reduced sensitivity to actin cytoskeleton disruptors compared to their controls. Consistently, actin filament dynamics, but not organization, were suppressed in TORC1-impaired cells. Subcellular localization analysis and quantification of ATP concentration demonstrated that RAPTOR1B localized at cytoplasm and mitochondria and that ATP levels were significantly reduced in TORC1-impaired plants. Further pharmacologic experiments showed that the inhibition of mitochondrial functions led to phenotypes mimicking those observed in raptor1b mutants at the level of both plant growth and actin dynamics. Exogenous feeding of adenine could partially restore ATP levels and actin dynamics in TORC1-deficient plants. Thus, these data support an important role for TORC1 in coordinating ATP homeostasis and actin dynamics in plant cells.

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Language(s): eng - English
 Dates: 2022-09-122022-09-12
 Publication Status: Published in print
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.1073/pnas.2122969119
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 119 (38) Sequence Number: e2122969119 Start / End Page: - Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230