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  Structure of a backtracked hexasomal intermediate of nucleosome transcription

Farnung, L., Ochmann, M., Garg, G., Vos, S., & Cramer, P. (2022). Structure of a backtracked hexasomal intermediate of nucleosome transcription. Molecular Cell, 82(17), 3126-3134.e7. doi:10.1016/j.molcel.2022.06.027.

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 Creators:
Farnung, Lucas1, Author           
Ochmann, Moritz1, Author           
Garg, Gaurika1, Author           
Vos, Seychelle1, Author           
Cramer, P.1, Author                 
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1Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350224              

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Free keywords: transcription RNA polymerase II RNA Pol II nucleosome hexasome nucleosome passage TFIIS transcription elongation H2A-H2B dimer backtracking chromatin nucleus
 Abstract: During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the help of various elongation
factors. Here, we show that RNA Pol II achieves efficient nucleosome passage when the human elongation
factors DSIF, PAF1 complex (PAF), RTF1, SPT6, and TFIIS are present. The cryo-EM structure of an
intermediate of the nucleosome passage shows a partially unraveled hexasome that lacks the proximal
H2A–H2B dimer and interacts with the RNA Pol II jaw, DSIF, and the CTR9trestle helix. RNA Pol II adopts a
backtracked state with the RNA 30 end dislodged from the active site and bound in the RNA Pol II pore. Additional
structures and biochemical data show that human TFIIS enters the RNA Pol II pore and stimulates the
cleavage of the backtracked RNA and nucleosome passage.

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Language(s): eng - English
 Dates: 2022-05-172022-03-302022-06-212022-07-192022-09-01
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.molcel.2022.06.027
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Title: Molecular Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: 17 Volume / Issue: 82 (17) Sequence Number: - Start / End Page: 3126 - 3134.e7 Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929