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  Determination of Sialic Acid Isomers from Released N-Glycans Using Ion Mobility Spectrometry

Manz, C., Mancera-Arteu, M., Zappe, A., Hanozin, E., Polewski, L., Giménez, E., et al. (2022). Determination of Sialic Acid Isomers from Released N-Glycans Using Ion Mobility Spectrometry. Analytical Chemistry, 94(39), 13323-13331. doi:10.1021/acs.analchem.2c00783.

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 Creators:
Manz, Christian1, Author           
Mancera-Arteu, Montserrat, Author
Zappe, Andreas, Author
Hanozin, Emeline1, Author           
Polewski, Lukasz1, Author           
Giménez, Estela, Author
Sanz-Nebot, Victoria, Author
Pagel, Kevin1, Author           
Affiliations:
1Molecular Physics, Fritz Haber Institute, Max Planck Society, ou_634545              

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Free keywords: Carbohydrates; Chemical biology; Chemical structure; Ions; Molecular structure
 Abstract: Complex carbohydrates are ubiquitous in nature and represent one of the major classes of biopolymers. They can exhibit highly diverse structures with multiple branched sites as well as a complex regio- and stereochemistry. A common way to analytically address this complexity is liquid chromatography (LC) in combination with mass spectrometry (MS). However, MS-based detection often does not provide sufficient information to distinguish glycan isomers. Ion mobility-mass spectrometry (IM-MS)─a technique that separates ions based on their size, charge, and shape─has recently shown great potential to solve this problem by identifying characteristic isomeric glycan features such as the sialylation and fucosylation pattern. However, while both LC-MS and IM-MS have clearly proven their individual capabilities for glycan analysis, attempts to combine both methods into a consistent workflow are lacking. Here, we close this gap and combine hydrophilic interaction liquid chromatography (HILIC) with IM-MS to analyze the glycan structures released from human alpha-1-acid glycoprotein (hAGP). HILIC separates the crude mixture of highly sialylated multi-antennary glycans, MS provides information on glycan composition, and IMS is used to distinguish and quantify α2,6- and α2,3-linked sialic acid isomers based on characteristic fragments. Further, the technique can support the assignment of antenna fucosylation. This feature mapping can confidently assign glycan isomers with multiple sialic acids within one LC-IM-MS run and is fully compatible with existing workflows for N-glycan analysis.

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Language(s): eng - English
 Dates: 2022-02-162022-09-052022-09-192022-10-04
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acs.analchem.2c00783
 Degree: -

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Title: Analytical Chemistry
  Abbreviation : Anal. Chem.
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: 9 Volume / Issue: 94 (39) Sequence Number: - Start / End Page: 13323 - 13331 Identifier: ISSN: 0003-2700
CoNE: https://pure.mpg.de/cone/journals/resource/111032812862552